@article{906abd37a6014bc49c31cf4bb6046d6a,
title = "Improved calcium imaging in transgenic mice expressing a troponin C-based biosensor",
abstract = "Fluorescent Ca2+ indicator proteins (FCIPs) are attractive tools for studying Ca2+ dynamics in live cells. Here we describe transgenic mouse lines expressing a troponin C (TnC)-based biosensor. The biosensor is widely expressed in neurons and has improved Ca2+ sensitivity both in vitro and in vivo. This allows FCIP-based two-photon Ca2+ imaging of distinct neurons and their dendrites in vivo, and opens a new avenue for structure-function analysis of intact neuronal circuits.",
author = "Nicola Heim and Olga Garaschuk and Friedrich, {Michael W.} and Marco Mank and Milos, {Ruxandra I.} and Yury Kovalchuk and Arthur Konnerth and Oliver Griesbeck",
note = "Funding Information: We thank M. B{\"o}sl for doing pronucleus injections, and A. Schulze-Schenke and S. Direnberger for assistance. Supported by grants of the Deutsche Forschungsgemeinschaft (SFB 391 and SFB 596) and the Bundesministerium f{\"u}r Bildung und Forschung (NGFN-2).",
year = "2007",
month = feb,
doi = "10.1038/nmeth1009",
language = "English",
volume = "4",
pages = "127--129",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Research",
number = "2",
}