@article{02e2a7410abe4841b0a8dbe83b7e0eb2,
title = "Importance of the intracellular domain of NR2 subunits for NMDA receptor function in vivo",
abstract = "NMDA receptors, a class of glutamate-gated cation channels with high Ca2+ conductance, mediate fast transmission and plasticity of central excitatory synapses. We show here that gene-targeted mice expressing NMDA receptors without the large intracellular C-terminal domain of any one of three NR2 subunits phenotypically resemble mice made deficient in that particular subunit. Mice expressing the NR2B subunit in a C-terminally truncated form (NR2B(ΔC/ΔC) mice) die perinatally. NR2A(ΔC/ΔC) mice are viable but exhibit impaired synaptic plasticity and contextual memory. These and NR2C(ΔC/ΔC) mice display deficits in motor coordination. C-terminal truncation of NR2 subunits does not interfere with the formation of gateable receptor channels that can be synaptically activated. Thus, the phenotypes of our mutants appear to reflect defective intracellular signaling.",
author = "Rolf Sprengel and Bettina Suchanek and Carla Amico and Rossella Brusa and Nail Burnashev and Andrei Rozov and O{\O}ivind Hvalby and Vidar Jensen and Ole Paulsen and Per Andersen and Kim, {Jeansok J.} and Thompson, {Richard F.} and William Sun and Webster, {Lorna C.} and Grant, {Seth G.N.} and Jens Eilers and Arthur Konnerth and Jianying Li and McNamara, {James O.} and Seeburg, {Peter H.}",
note = "Funding Information: We thank Dr. I. Bartke for NR2 subunit antibodies; Dr. J. H. Morrison for antibody 54.1 to NR1; and A. Herold, U. Amtmann, and N. Marandi for help with experiments. This work was supported, in part, by the Norwegian Research Council grant 111276/310 to P. A.; National Institutes of Health grant IF32MN10521–01 Behavioral Neuroscience Review to J. J. K.; National Institute on Aging grant AG0093 to W. S.; National Science Foundation grant IBN9215069 and National Institutes of Health grant AF05142 to R. F. T.; the Wellcome Trust to S. G. N. G.; Deutsche Forschungsgemeinschaft Programm grant in Schwerpunktsprogramm Molecular Physiology to A. K.; National Institutes of Disorders and Stroke grant NS32334 to J. O. M.; and Deutsche Forschungsgemeinschaft grant Sondersforschungbereich 319/B9 and Funds of the German Chemical Industry to P. H. S.",
year = "1998",
month = jan,
day = "23",
doi = "10.1016/S0092-8674(00)80921-6",
language = "English",
volume = "92",
pages = "279--289",
journal = "Cell",
issn = "0092-8674",
publisher = "Elsevier B.V.",
number = "2",
}