Abstract
The influence of different influent substrates on biomass protein composition was examined by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Activated sludge from six sequencing batch reactors (SBRs) was investigated; four reactors were fed with model substrates and two received effluents from a wood milling process and a paper production process, respectively. Our investigations showed that in 2D-PAGE complex substrates caused a less diverse protein pattern than model wastewater composed of simple and low molecular weight compounds. This may be caused by complex formation by high molecular weight compounds of substrate with proteins. A more likely explanation is the presence of a more diversified microbial population resulting in a lower concentration of individual proteins, so that detection limits after staining were too high to observe discrete spots.
| Original language | English |
|---|---|
| Pages (from-to) | 363-366 |
| Number of pages | 4 |
| Journal | Water Science and Technology |
| Volume | 37 |
| Issue number | 4-5 |
| DOIs | |
| State | Published - 1998 |
| Event | Proceedings of the 1997 2nd International Conference on Microorganisms in Activated Sludge and Biofilm Processes - Berkeley, CA, USA Duration: 21 Jul 1997 → 23 Jul 1997 |
Keywords
- Activated sludge
- Microbial polymers
- Paper mill wastewater
- Proteins
- Sequencing batch reactor (SBR)
- Two-dimensional gel electrophoresis (2D-PAGE)