Impact of different substrates on biomass protein composition during wastewater treatment investigated by two-dimensional electrophoresis

Sylvia Huber, Sabine Minnebusch, Stefan Wuertz, Peter A. Wilderer, Brigitte Helmreich

Research output: Contribution to journalConference articlepeer-review

9 Scopus citations

Abstract

The influence of different influent substrates on biomass protein composition was examined by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Activated sludge from six sequencing batch reactors (SBRs) was investigated; four reactors were fed with model substrates and two received effluents from a wood milling process and a paper production process, respectively. Our investigations showed that in 2D-PAGE complex substrates caused a less diverse protein pattern than model wastewater composed of simple and low molecular weight compounds. This may be caused by complex formation by high molecular weight compounds of substrate with proteins. A more likely explanation is the presence of a more diversified microbial population resulting in a lower concentration of individual proteins, so that detection limits after staining were too high to observe discrete spots.

Original languageEnglish
Pages (from-to)363-366
Number of pages4
JournalWater Science and Technology
Volume37
Issue number4-5
DOIs
StatePublished - 1998
EventProceedings of the 1997 2nd International Conference on Microorganisms in Activated Sludge and Biofilm Processes - Berkeley, CA, USA
Duration: 21 Jul 199723 Jul 1997

Keywords

  • Activated sludge
  • Microbial polymers
  • Paper mill wastewater
  • Proteins
  • Sequencing batch reactor (SBR)
  • Two-dimensional gel electrophoresis (2D-PAGE)

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