Immunolocalization of non-extractable (bound) residues of pesticides and industrial contaminants in plants and soil

Andrea Dankwardt, Bertold Hock

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

The application of immunochemical methods for the investigation of non-extractable (bound) residues is reviewed. Non-extractable residues may be presented to antibodies as antigenic determinants, which are exposed for instance in plant tissue and humic substances. Fluorescent probes as well as enzyme markers have been applied for the detection of bound residues. The application of antibodies labeled with fluorescein isothiocyanate (FITC) and phycoerythrin revealed the presence of atrazine in cryosections of atrazine- treated corn leaves and water plants. Atrazine could be localized by antibodies coupled to fluorescent markers in soil from corn fields but not in atrazine-free soil. Quantitative results were obtained by the application of enzyme immunoassays to the investigation of triazine and 2,4,6-trinitro-toluene (TNT) residues, bound to soil humic acids. Finally, the use of antibodies with different recognition patterns provides information on the ligation of non-extractable residues to the matrix.

Original languageEnglish
Pages (from-to)523-533
Number of pages11
JournalChemosphere
Volume45
Issue number4-5
DOIs
StatePublished - 2001
Externally publishedYes

Keywords

  • Antibodies
  • Atrazine
  • Fluorescence labeling
  • Immunoassay
  • TNT

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