Abstract
Using immunohistochemistry we studied the presence of calbindin in myenteric neurones of the guinea-pig stomach. A rabbit anti recombinant rat calbindin-D28k (CALB) stained 12, 12 and 25% of all myenteric neurones in the fundus, corpus and antrum, respectively. A rabbit anti recombinant human CALB stained 4, 4 and 16%, respectively. A mouse monoclonal antibody against the chicken intestinal CALB showed no labelling. In all regions most calbindin neurones were additionally choline acetyltransferase (CHAT) positive while only a small proportion exhibited nicotinamide adenosine dinucleatide phosphate (NADPH)-diaphorase-activity. Numerous calbindin -positive varicose nerve fibres were present within myenteric ganglia, rarely detectable in the muscle layers and virtually absent in the mucosa. This study demonstrated that a subpopulation of cholinergic myenteric neurones in the stomach contain calbindin and suggested that many of these neurones fulfil interneuronal tasks.
| Original language | English |
|---|---|
| Pages (from-to) | 71-74 |
| Number of pages | 4 |
| Journal | Neuroscience Letters |
| Volume | 270 |
| Issue number | 2 |
| DOIs | |
| State | Published - 30 Jul 1999 |
| Externally published | Yes |
Keywords
- Acetylcholine
- Calbindin
- Calcium-binding proteins
- Enteric nervous system
- Interneurones
- Myenteric plexus
- Nitric oxide
- Stomach