Immunochemical studies with synthetic immunogens chemically related to peptidoglycan

P. H. Seidl, K. H. Schleifer

Research output: Contribution to journalArticlepeer-review

Abstract

Rabbits were immunized with synthetic immunogens human serum albumin (HSA) (Gly L Ala L Ala D Ala D Ala)39 and HSA (Gly γ D Glu L Ala D Ala D Ala)40, respectively. Antibodies against HSA (Gly L Ala L Ala D Ala D Ala)39 showed a strong precipitin reaction with the homologous antigen, with HSA (Gly γ D Glu L Ala D Ala D Ala)40 and with solubilized peptidoglycan containing peptide subunits with C terminal D alanyl D alanine. The albumin peptide conjugates also cross reacted with rabbit antisera to Streptococcus A variant, which contain antibodies directed against the peptide moiety of peptidoglycan. The proof for identical determinant groups of peptidoglycan of Streptococcus A variant and HSA (Gly L Ala L Ala D Ala D Ala)39 was furnished by Ouchterlony gel diffusion studies and by the appropriate inhibition tests of the quantitative precipitin reaction. Immunization of rabbits with HSA (Gly γ D Glu L Ala D Ala D Ala)40 yielded antisera which, besides the specificity of antisera to HSA (Gly L Ala L Ala D Ala D Ala)39, showed an additional specificity.

Original languageEnglish
Pages (from-to)157-164
Number of pages8
JournalZeitschrift fur Immunitatsforschung
Volume149
Issue number2-4
StatePublished - 1975
Externally publishedYes

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