Immunochemical array for the identification of cross-reacting analytes

Michael Winklmair, Andreas J. Schuetz, Michael G. Weller, Reinhard Niessner

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

A method for the identification and quantification of cross-reacting analytes using competitive immunochemical assays is described. The method uses information both of antibodies with and without significant inhibition caused by a sample. Maximum concentrations of all possible analytes were estimated for all antibodies not showing a significant inhibition. These maximum concentrations could be used to exclude certain analytes from the further identification process. A minimum variance method was used for the identification of analytes from the data given by antibodies showing significant inhibition. All samples were measured in a parallel affinity sensor array (PASA). The PASA system allows the parallel performance of numerous individual immunochemical assays. Triazines were used as a model substance class. Samples containing either atrazine, terbuthylazine, simazine or deethylatrazine at different concentration levels were generated and analyzed in the PASA system. 11 out of 13 samples were correctly identified, 2 samples could not be identified without doubt, no wrong identification was observed. Samples of atrazine, terbuthylazine and simazine at a concentration level of 0.1 μg/L, the EU maximum admissible concentration for individual pesticides, and of deethylatrazine at 0.3 μg/L could be quantified.

Original languageEnglish
Pages (from-to)731-737
Number of pages7
JournalFresenius' Journal of Analytical Chemistry
Volume363
Issue number8
DOIs
StatePublished - 1999

Fingerprint

Dive into the research topics of 'Immunochemical array for the identification of cross-reacting analytes'. Together they form a unique fingerprint.

Cite this