Immune cell imaging using multi-spectral optoacoustic tomography

Stratis Tzoumas, Angelika Zaremba, Uwe Klemm, Antonio Nunes, Karin Schaefer, Vasilis Ntziachristos

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Multispectral optoacoustic tomography (MSOT) offers the potential to image in high-resolution cells tagged with optical labels. In contrast to single wavelength imaging, multispectral excitation and spectral unmixing can differentiate labeled moieties over tissue absorption in the absence of background measurements. This feature can enable longitudinal cellular biology studies well beyond the depths reached by optical microscopy. However, the relation between spectrally resolved fluorescently labeled cells and optoacoustic detection has not been systematically investigated. Herein, we measured titrations of fluorescently labeled cells and establish the optoacoustic signal generated by these cells as a function of cell number and across different cell types. We then assess the MSOT sensitivity to resolve cells implanted in animals.

Original languageEnglish
Pages (from-to)3523-3526
Number of pages4
JournalOptics Letters
Volume39
Issue number12
DOIs
StatePublished - 15 Jun 2014
Externally publishedYes

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