Immobilization of PETase enzymes on magnetic iron oxide nanoparticles for the decomposition of microplastic PET

Sebastian P. Schwaminger; Stefan Fehn; Tobias Steegmüller; Stefan Rauwolf; Hannes Löwe; Katharina Pflüger-Grau; Sonja Berensmeier

doi:10.1039/d1na00243k

Immobilization of PETase enzymes on magnetic iron oxide nanoparticles for the decomposition of microplastic PET

Sebastian P. Schwaminger, Stefan Fehn, Tobias Steegmüller, Stefan Rauwolf, Hannes Löwe, Katharina Pflüger-Grau, Sonja Berensmeier

Chair of Bioseparation Engineering

Technical University of Munich

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

Polyethylene terephthalate (PET) is responsible for a large amount of environmental contamination with microplastics. Based on its high affinity, the PET degrading enzyme PETase can be immobilized on superparamagnetic iron oxide nanoparticles through a His-tag. The His-tag increases enzyme stability, and allows magnetic separation for recovery. Multiple recycling steps are possible and microplastic particles can be decomposed depending on the PET's crystallinity. The separation or decomposition of PET allows for a sustainable way to remove microplastic from water.

Original language	English
Pages (from-to)	4395-4399
Number of pages	5
Journal	Nanoscale Advances
Volume	3
Issue number	15
DOIs	https://doi.org/10.1039/d1na00243k
State	Published - 7 Aug 2021

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abstract = "Polyethylene terephthalate (PET) is responsible for a large amount of environmental contamination with microplastics. Based on its high affinity, the PET degrading enzyme PETase can be immobilized on superparamagnetic iron oxide nanoparticles through a His-tag. The His-tag increases enzyme stability, and allows magnetic separation for recovery. Multiple recycling steps are possible and microplastic particles can be decomposed depending on the PET's crystallinity. The separation or decomposition of PET allows for a sustainable way to remove microplastic from water.",

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AU - Schwaminger, Sebastian P.

AU - Fehn, Stefan

AU - Steegmüller, Tobias

AU - Rauwolf, Stefan

AU - Löwe, Hannes

AU - Pflüger-Grau, Katharina

AU - Berensmeier, Sonja

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AB - Polyethylene terephthalate (PET) is responsible for a large amount of environmental contamination with microplastics. Based on its high affinity, the PET degrading enzyme PETase can be immobilized on superparamagnetic iron oxide nanoparticles through a His-tag. The His-tag increases enzyme stability, and allows magnetic separation for recovery. Multiple recycling steps are possible and microplastic particles can be decomposed depending on the PET's crystallinity. The separation or decomposition of PET allows for a sustainable way to remove microplastic from water.

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JO - Nanoscale Advances

JF - Nanoscale Advances

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ER -

Immobilization of PETase enzymes on magnetic iron oxide nanoparticles for the decomposition of microplastic PET

Abstract

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