Immobilization of horseradish peroxidase via an amino silane on oxidized ultrananocrystalline diamond

Jorge Hernando, Tahmineh Pourrostami, Jose A. Garrido, Oliver A. Williams, Dieter M. Gruen, Alexander Kromka, Doris Steinmüller, Martin Stutzmann

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

We discuss the complete functionalization of nitrogen-doped ultrananocrystalline diamond (UNCD) films, starting from an oxidized surface. First, the presence of hydroxyl groups on oxidized nanocrystalline diamond (NCD) was confirmed by fluorescence microscopy. Next, the grafting of a linker molecule such as 3-aminopropylmethyldiethoxysilane on oxidized NCD was confirmed by fluorescence microscopy and X-ray photoelectron spectroscopy (XPS). Then the horseradish peroxidase (HRP) enzyme was immobilized on silane-modified initially oxidized UNCD. The HRP-modified UNCD was characterized by electrochemical techniques, such as faradaic cyclic voltammetry and the amperometric response to H2O2. This response to H2O2 is discussed in terms of the layer-by-layer configuration used and the electronic properties of conducting UNCD.

Original languageEnglish
Pages (from-to)138-143
Number of pages6
JournalDiamond and Related Materials
Volume16
Issue number1
DOIs
StatePublished - Jan 2007

Keywords

  • Biomaterials
  • Diamond film
  • Electrochemical
  • Nanocrystalline

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