Imaging the distribution of photoswitchable probes with temporally-unmixed multispectral optoacoustic tomography

X. Luis Dean-Ben, Andre C. Stiel, Yuanyuan Jiang, Vasilis Ntziachristos, Gil G. Westmeyer, Daniel Razansky

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Synthetic and genetically encoded chromo-and fluorophores have become indispensable tools for biomedical research enabling a myriad of applications in imaging modalities based on biomedical optics. The versatility offered by the optoacoustic (photoacoustic) contrast mechanism enables to detect signals from any substance absorbing light, and hence these probes can be used as optoacoustic contrast agents. While contrast versatility generally represents an advantage of optoacoustics, the strong background signal generated by light absorption in endogeneous chromophores hampers the optoacoustic capacity to detect a photo-absorbing agent of interest. Increasing the optoacoustic sensitivity is then determined by the capability to differentiate specific features of such agent. For example, multispectral optoacoustic tomography (MSOT) exploits illuminating the tissue at multiple optical wavelengths to spectrally resolve (unmix) the contribution of different chromophores. Herein, we present an alternative approach to enhance the sensitivity and specificity in the detection of optoacoustic contrast agents. This is achieved with photoswitchable probes that change optical absorption upon illumination with specific optical wavelengths. Thereby, temporally unmixed MSOT (tuMSOT) is based on photoswitching the compounds according to defined schedules to elicit specific time-varying optoacoustic signals, and then use temporal unmixing algorithms to locate the contrast agent based on their particular temporal profile. The photoswitching kinetics is further affected by light intensity, so that tuMSOT can be employed to estimate the light fluence distribution in a biological sample. The performance of the method is demonstrated herein with the reversibly switchable fluorescent protein Dronpa and its fast-switching fatigue resistant variant Dronpa-M159T.

Original languageEnglish
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2016
EditorsAlexander A. Oraevsky, Lihong V. Wang
PublisherSPIE
ISBN (Electronic)9781628419429
DOIs
StatePublished - 2016
EventPhotons Plus Ultrasound: Imaging and Sensing 2016 - San Francisco, United States
Duration: 14 Feb 201617 Feb 2016

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9708
ISSN (Print)1605-7422

Conference

ConferencePhotons Plus Ultrasound: Imaging and Sensing 2016
Country/TerritoryUnited States
CitySan Francisco
Period14/02/1617/02/16

Keywords

  • Dronpa
  • Dronpa-M159T
  • Optoacoustic imaging
  • photoswitchable proteins
  • temporally unmixed multispectral optoacoustic tomography

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