Imaging-Based Screening Platform Assists Protein Engineering

Arne Fabritius; David Ng; Andreas Michael Kist; Mutlu Erdogan; Ruben Portugues; Oliver Griesbeck

doi:10.1016/j.chembiol.2018.08.008

Imaging-Based Screening Platform Assists Protein Engineering

Arne Fabritius, David Ng, Andreas Michael Kist, Mutlu Erdogan, Ruben Portugues, Oliver Griesbeck

Max Planck Institute of Neurobiology

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Protein evolution requires generation and screening of large numbers of variants. To expedite screening, Fabritius et al. built a platform that integrates fluorescence-based image analysis and picking of bacterial colonies. They engineered mCarmine, a far-red-emitting fluorescent protein for tagging and deep tissue imaging.

Original language	English
Pages (from-to)	1554-1561.e8
Journal	Cell Chemical Biology
Volume	25
Issue number	12
DOIs	https://doi.org/10.1016/j.chembiol.2018.08.008
State	Published - 20 Dec 2018
Externally published	Yes

Keywords

directed evolution
fluorescent proteins
image analysis
protein engineering
screening

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10.1016/j.chembiol.2018.08.008

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title = "Imaging-Based Screening Platform Assists Protein Engineering",

abstract = "Protein evolution requires generation and screening of large numbers of variants. To expedite screening, Fabritius et al. built a platform that integrates fluorescence-based image analysis and picking of bacterial colonies. They engineered mCarmine, a far-red-emitting fluorescent protein for tagging and deep tissue imaging.",

keywords = "directed evolution, fluorescent proteins, image analysis, protein engineering, screening",

author = "Arne Fabritius and David Ng and Kist, {Andreas Michael} and Mutlu Erdogan and Ruben Portugues and Oliver Griesbeck",

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AU - Ng, David

AU - Kist, Andreas Michael

AU - Erdogan, Mutlu

AU - Portugues, Ruben

AU - Griesbeck, Oliver

PY - 2018/12/20

Y1 - 2018/12/20

N2 - Protein evolution requires generation and screening of large numbers of variants. To expedite screening, Fabritius et al. built a platform that integrates fluorescence-based image analysis and picking of bacterial colonies. They engineered mCarmine, a far-red-emitting fluorescent protein for tagging and deep tissue imaging.

AB - Protein evolution requires generation and screening of large numbers of variants. To expedite screening, Fabritius et al. built a platform that integrates fluorescence-based image analysis and picking of bacterial colonies. They engineered mCarmine, a far-red-emitting fluorescent protein for tagging and deep tissue imaging.

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KW - fluorescent proteins

KW - image analysis

KW - protein engineering

KW - screening

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SN - 2451-9456

VL - 25

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JO - Cell Chemical Biology

JF - Cell Chemical Biology

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Imaging-Based Screening Platform Assists Protein Engineering

Abstract

Keywords

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