Image analysis for calculation of the toxicity degree of cells in phase contrast microscopy images

M. Athelogou; M. Eblenkamp; G. Schmidt; F. Novotny; E. Wintermantel; G. Binnig

doi:10.1007/978-3-642-19335-4_29

Image analysis for calculation of the toxicity degree of cells in phase contrast microscopy images

M. Athelogou, M. Eblenkamp, G. Schmidt, F. Novotny, E. Wintermantel, G. Binnig

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

Because of the very special type of contrast in phase-contrast images, it is almost impossible to perform fully automated single-cell analysis and quantification successfully. Because fluorescent dyes are highly toxic, phase-contrast images are commonly used to monitor live cells. In this paper, we present a method for the fully automated segmentation, classification and quantification of individual cell morphology in phase-contrast images. We calculate the confluence of the cell population and quantify the degree of toxic damage to each individual cell following phenol incubation. The results are then compared to standard cytotoxicity assays.

Original language	English
Title of host publication	Bildverarbeitung fur die Medizin 2011
Subtitle of host publication	Algorithmen - Systeme - Anwendungen, BVM 2011 - Proceedings des Workshops
Pages	134-138
Number of pages	5
DOIs	https://doi.org/10.1007/978-3-642-19335-4_29
State	Published - 2011
Event	Workshops Bildverarbeitung fur die Medizin: Algorithmen - Systeme - Anwendungen, BVM 2011 - Workshop on Image Processing for Medicine: Algorithms - Systems - Applications, BVM 2011 - Lubeck, Germany Duration: 20 Mar 2011 → 22 Mar 2011

Publication series

Name	Informatik aktuell
ISSN (Print)	1431-472X

Conference

Conference	Workshops Bildverarbeitung fur die Medizin: Algorithmen - Systeme - Anwendungen, BVM 2011 - Workshop on Image Processing for Medicine: Algorithms - Systems - Applications, BVM 2011
Country/Territory	Germany
City	Lubeck
Period	20/03/11 → 22/03/11

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10.1007/978-3-642-19335-4_29

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Athelogou, M., Eblenkamp, M., Schmidt, G., Novotny, F., Wintermantel, E., & Binnig, G. (2011). Image analysis for calculation of the toxicity degree of cells in phase contrast microscopy images. In Bildverarbeitung fur die Medizin 2011: Algorithmen - Systeme - Anwendungen, BVM 2011 - Proceedings des Workshops (pp. 134-138). (Informatik aktuell). https://doi.org/10.1007/978-3-642-19335-4_29

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title = "Image analysis for calculation of the toxicity degree of cells in phase contrast microscopy images",

abstract = "Because of the very special type of contrast in phase-contrast images, it is almost impossible to perform fully automated single-cell analysis and quantification successfully. Because fluorescent dyes are highly toxic, phase-contrast images are commonly used to monitor live cells. In this paper, we present a method for the fully automated segmentation, classification and quantification of individual cell morphology in phase-contrast images. We calculate the confluence of the cell population and quantify the degree of toxic damage to each individual cell following phenol incubation. The results are then compared to standard cytotoxicity assays.",

author = "M. Athelogou and M. Eblenkamp and G. Schmidt and F. Novotny and E. Wintermantel and G. Binnig",

year = "2011",

doi = "10.1007/978-3-642-19335-4_29",

language = "English",

isbn = "9783642193347",

series = "Informatik aktuell",

pages = "134--138",

booktitle = "Bildverarbeitung fur die Medizin 2011",

note = "Workshops Bildverarbeitung fur die Medizin: Algorithmen - Systeme - Anwendungen, BVM 2011 - Workshop on Image Processing for Medicine: Algorithms - Systems - Applications, BVM 2011 ; Conference date: 20-03-2011 Through 22-03-2011",

}

Athelogou, M, Eblenkamp, M, Schmidt, G, Novotny, F, Wintermantel, E & Binnig, G 2011, Image analysis for calculation of the toxicity degree of cells in phase contrast microscopy images. in Bildverarbeitung fur die Medizin 2011: Algorithmen - Systeme - Anwendungen, BVM 2011 - Proceedings des Workshops. Informatik aktuell, pp. 134-138, Workshops Bildverarbeitung fur die Medizin: Algorithmen - Systeme - Anwendungen, BVM 2011 - Workshop on Image Processing for Medicine: Algorithms - Systems - Applications, BVM 2011, Lubeck, Germany, 20/03/11. https://doi.org/10.1007/978-3-642-19335-4_29

Image analysis for calculation of the toxicity degree of cells in phase contrast microscopy images. / Athelogou, M.; Eblenkamp, M.; Schmidt, G. et al.
Bildverarbeitung fur die Medizin 2011: Algorithmen - Systeme - Anwendungen, BVM 2011 - Proceedings des Workshops. 2011. p. 134-138 (Informatik aktuell).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Athelogou, M.

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AU - Wintermantel, E.

AU - Binnig, G.

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Y2 - 20 March 2011 through 22 March 2011

ER -

Image analysis for calculation of the toxicity degree of cells in phase contrast microscopy images

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