Abstract
Wheat-dependent exercise-induced anaphylaxis (WDEIA) is characterized by anaphylactic reactions after wheat ingestion and physical exercise. IgE antibodies to recombinant ω5-gliadin are detectable in a majority of WDEIA patients, but other wheat allergens may also play a role in elicitation of WDEIA. Here, we performed a comprehensive analysis of IgE reactivity to different wheat proteins in 17 patients with confirmed WDEIA by ImmunoCAP research prototypes and a semi-quantitative microarray immunoassay with α/β/γ-gliadin, high-molecular-weight (HMW) glutenin, alpha-amylase inhibitor (AAI) dimer, and wheat lipid transfer protein (LTP). By ImmunoCAP, IgE to recombinant ω5-gliadin was detectable in 14/17 patients (82%), to α/β/γ-gliadin in 82% including the three patients lacking IgE to ω5-gliadin, and to HMW glutenin in 59%. The microarray revealed specifically γ-gliadin as the second most important allergen. These results demonstrate the additional diagnostic value of α/β- and γ-gliadin in particular in ω5- gliadin-negative patients in the diagnosis of WDEIA.
Original language | English |
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Pages (from-to) | 1457-1460 |
Number of pages | 4 |
Journal | Allergy: European Journal of Allergy and Clinical Immunology |
Volume | 67 |
Issue number | 11 |
DOIs | |
State | Published - Nov 2012 |
Externally published | Yes |
Keywords
- IgE
- gliadin
- recombinant allergens
- wheat allergy
- wheat-dependent exercise-induced anaphylaxis