TY - JOUR
T1 - IFITM/mil/fragilis family proteins IFITM1 and IFITM3 play distinct roles in mouse primordial germ cell homing and repulsion
AU - Tanaka, Satomi S.
AU - Yamaguchi, Yasuka L.
AU - Tsoi, Bonny
AU - Lickert, Heiko
AU - Tam, Patrick P.L.
N1 - Funding Information:
We thank Peter Rowe, David Loebel, and Samara Lewis for comments on the manuscript; Toru Nakano for the Pgc7 probe and anti-PGC7 antibody; Hiroshi Hamada for the Oct-3/4 probe; and Christine Smyth and Axel Neumann for technical help with microscopy. S.S.T. is supported by a Fellowship of the Japan Society for the Promotion of Science. P.P.L.T. is a Senior Principal Research Fellow of the National Health and Medical Research Council of Australia.
PY - 2005/12
Y1 - 2005/12
N2 - The family of interferon-induced transmembrane protein (Ifitm/mil/ fragilis) genes encodes cell surface proteins that may modulate cell adhesion and influence cell differentiation. Mouse Ifitm1 and -3, which are expressed in primordial germ cells (PGCs), are implicated to have roles in germ cell development, but the specific functions have been unclear. Our results show that Ifitm1 activity is required for PGC transit from the mesoderm into the endoderm, and that it appears to act via a repulsive mechanism, such that PGCs avoid Ifitm1-expressing tissues. In contrast, Ifitm3, which is expressed in migratory PGCs, is sufficient to confer autonomous PGC-like homing properties to somatic cells. These guidance activities are mediated by the N-terminal extracellular domain of the specific IFITM, which cannot be substituted by that of another family member. Complex homo- and/or heterotypic intercellular interactions among various IFITMs in PGCs and neighboring cells may underpin coordinated germ cell guidance in mice.
AB - The family of interferon-induced transmembrane protein (Ifitm/mil/ fragilis) genes encodes cell surface proteins that may modulate cell adhesion and influence cell differentiation. Mouse Ifitm1 and -3, which are expressed in primordial germ cells (PGCs), are implicated to have roles in germ cell development, but the specific functions have been unclear. Our results show that Ifitm1 activity is required for PGC transit from the mesoderm into the endoderm, and that it appears to act via a repulsive mechanism, such that PGCs avoid Ifitm1-expressing tissues. In contrast, Ifitm3, which is expressed in migratory PGCs, is sufficient to confer autonomous PGC-like homing properties to somatic cells. These guidance activities are mediated by the N-terminal extracellular domain of the specific IFITM, which cannot be substituted by that of another family member. Complex homo- and/or heterotypic intercellular interactions among various IFITMs in PGCs and neighboring cells may underpin coordinated germ cell guidance in mice.
UR - http://www.scopus.com/inward/record.url?scp=28444479846&partnerID=8YFLogxK
U2 - 10.1016/j.devcel.2005.10.010
DO - 10.1016/j.devcel.2005.10.010
M3 - Article
C2 - 16326387
AN - SCOPUS:28444479846
SN - 1534-5807
VL - 9
SP - 745
EP - 756
JO - Developmental Cell
JF - Developmental Cell
IS - 6
ER -