Identification of the arabidopsis thaliana flavonoid 3'-hydroxylase gene and functional expression of the encoded P450 enzyme

C. Schoenbohm, S. Martens, C. Eder, G. Forkmann, B. Weisshaar

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193 Scopus citations

Abstract

The phenylpropanoid pathway results in the synthesis of thousands of compounds, including flavonoids like flavonols, anthocyanidins and tannins. In Arabidopsis thaliana, the lack of tannins in the seed coat (testa) causes the transparent testa (tt) phenotype. In the present study, we identified the gene responsible for the tt7 mutation. We show that TT7 encodes the enzyme flavonoid 3'-hydroxylase (F3'H), and demonstrate that this P450-dependent monooxygenase has F3'H activity. The availability of the AtF3'H gene and promoter sequence will allow us to study the coregulation of a complete set of flavonol and anthocyanidin biosynthesis genes in A. thaliana, and makes in vitro synthesis of hydroxylated flavonoids more feasible.

Original languageEnglish
Pages (from-to)749-753
Number of pages5
JournalBiological Chemistry
Volume381
Issue number8
DOIs
StatePublished - 2000
Externally publishedYes

Keywords

  • Arabidopsis
  • Flavonoid biosynthesis
  • P450 monooxygenase
  • Transparent testa
  • Tt7

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