Identification of dopamine receptor in tetrahymena thermophila by fluorescent ligands

Asad Ud-Daula, Gerd Pfister, Karl Werner Schramm

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Two types dopamine receptor present in the cell membrane of vertebrates. But in this study D1 receptor was identified in the invertebrate ciliates protozoan, Tetrahymena thermop0hila by use of fluorescent ligands. D1 specific agonist SKF-38393 binds specifically to Tetrahymena. The specific binding of SKF-38393 was encountered by equimolar addition of D1 antagonist thus showed no binding of ligands. In addition, it was also proved that the D1 specific agonist did not cross bind with the D2 type receptor due to the equimolar addition of D2 selective antagonist spiperone. Interestingly this study also showed that the dopamine receptor present in the endoplasmic reticulum and endosomes of Tetrahymena as well as cell membrane which was revealed by laser scanning microscope. Therefore, this evidence supports the existence of a D1 receptor in the ciliate protozoan.

Original languageEnglish
Pages (from-to)1133-1138
Number of pages6
JournalPakistan Journal of Biological Sciences
Volume15
Issue number23
DOIs
StatePublished - 2012

Keywords

  • Agonist
  • Antagonist
  • Ciliates protozoan
  • Dopamine receptors
  • Fluorescent ligands
  • LMS
  • SKF-38393
  • Tetrahymena thermophila

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