Identification of differentially expressed proteins in triple-negative breast carcinomas using DIGE and mass spectrometry

Daniela M. Schulz; Claudia Böllner; Gerry Thomas; Mike Atkinson; Irene Esposito; Heinz Höfler; Michaela Aubele

doi:10.1021/pr900071h

Identification of differentially expressed proteins in triple-negative breast carcinomas using DIGE and mass spectrometry

Daniela M. Schulz
, Claudia Böllner
, Gerry Thomas
, Mike Atkinson
, Irene Esposito
, Heinz Höfler
, Michaela Aubele

Helmholtz Zentrum München German Research Center for Environmental Health
Hammersmith Hospital
Technical University of Munich

Research output: Contribution to journal › Article › peer-review

53 Scopus citations

Abstract

We compared the protein expression pattern of triple-negative breast carcinomas (HER2-, ER-, PR-) versus those being positive for HER2 and negative for the hormone receptors (HER2+, ER-, PR-) by 2-D DIGE and mass spectrometry. We obtained differential expression patterns for several glycolytic enzymes (as for example MDH2, PGK1, TKT, Aldolase1), cytokeratins (CK7, 8, 9, 14, 17, 19), further structure proteins (vimentin, fibronectin, L-plastin), for NME1-NME2, lactoferrin, and members of the Annexin family. Western blot analysis and immunohistochemistry were conducted to verify the results. The identified marker proteins may advance a more detailed characterization of triple-negative breast cancers and may contribute to the development of better treatment strategies.

Original language	English
Pages (from-to)	3430-3438
Number of pages	9
Journal	Journal of Proteome Research
Volume	8
Issue number	7
DOIs	https://doi.org/10.1021/pr900071h
State	Published - 6 Jul 2009

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

2D-DIGE
Breast cancer
MALDI-TOF/TOFMS
Proteomics
Triple-negative

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10.1021/pr900071h

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title = "Identification of differentially expressed proteins in triple-negative breast carcinomas using DIGE and mass spectrometry",

abstract = "We compared the protein expression pattern of triple-negative breast carcinomas (HER2-, ER-, PR-) versus those being positive for HER2 and negative for the hormone receptors (HER2+, ER-, PR-) by 2-D DIGE and mass spectrometry. We obtained differential expression patterns for several glycolytic enzymes (as for example MDH2, PGK1, TKT, Aldolase1), cytokeratins (CK7, 8, 9, 14, 17, 19), further structure proteins (vimentin, fibronectin, L-plastin), for NME1-NME2, lactoferrin, and members of the Annexin family. Western blot analysis and immunohistochemistry were conducted to verify the results. The identified marker proteins may advance a more detailed characterization of triple-negative breast cancers and may contribute to the development of better treatment strategies.",

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author = "Schulz, \{Daniela M.\} and Claudia B{\"o}llner and Gerry Thomas and Mike Atkinson and Irene Esposito and Heinz H{\"o}fler and Michaela Aubele",

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doi = "10.1021/pr900071h",

language = "English",

volume = "8",

pages = "3430--3438",

journal = "Journal of Proteome Research",

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TY - JOUR

T1 - Identification of differentially expressed proteins in triple-negative breast carcinomas using DIGE and mass spectrometry

AU - Schulz, Daniela M.

AU - Böllner, Claudia

AU - Thomas, Gerry

AU - Atkinson, Mike

AU - Esposito, Irene

AU - Höfler, Heinz

AU - Aubele, Michaela

PY - 2009/7/6

Y1 - 2009/7/6

N2 - We compared the protein expression pattern of triple-negative breast carcinomas (HER2-, ER-, PR-) versus those being positive for HER2 and negative for the hormone receptors (HER2+, ER-, PR-) by 2-D DIGE and mass spectrometry. We obtained differential expression patterns for several glycolytic enzymes (as for example MDH2, PGK1, TKT, Aldolase1), cytokeratins (CK7, 8, 9, 14, 17, 19), further structure proteins (vimentin, fibronectin, L-plastin), for NME1-NME2, lactoferrin, and members of the Annexin family. Western blot analysis and immunohistochemistry were conducted to verify the results. The identified marker proteins may advance a more detailed characterization of triple-negative breast cancers and may contribute to the development of better treatment strategies.

AB - We compared the protein expression pattern of triple-negative breast carcinomas (HER2-, ER-, PR-) versus those being positive for HER2 and negative for the hormone receptors (HER2+, ER-, PR-) by 2-D DIGE and mass spectrometry. We obtained differential expression patterns for several glycolytic enzymes (as for example MDH2, PGK1, TKT, Aldolase1), cytokeratins (CK7, 8, 9, 14, 17, 19), further structure proteins (vimentin, fibronectin, L-plastin), for NME1-NME2, lactoferrin, and members of the Annexin family. Western blot analysis and immunohistochemistry were conducted to verify the results. The identified marker proteins may advance a more detailed characterization of triple-negative breast cancers and may contribute to the development of better treatment strategies.

KW - 2D-DIGE

KW - Breast cancer

KW - MALDI-TOF/TOFMS

KW - Proteomics

KW - Triple-negative

UR - https://www.scopus.com/pages/publications/67650388533

U2 - 10.1021/pr900071h

DO - 10.1021/pr900071h

M3 - Article

C2 - 19485423

AN - SCOPUS:67650388533

SN - 1535-3893

VL - 8

SP - 3430

EP - 3438

JO - Journal of Proteome Research

JF - Journal of Proteome Research

IS - 7

ER -

Identification of differentially expressed proteins in triple-negative breast carcinomas using DIGE and mass spectrometry

Abstract

UN SDGs

Keywords

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