Identification of Bitter-Taste Intensity and Molecular Weight as Amino Acid Determinants for the Stimulating Mechanisms of Gastric Acid Secretion in Human Parietal Cells in Culture

Verena Stoeger; Kathrin I. Liszt; Barbara Lieder; Martin Wendelin; Muhammet Zopun; Joachim Hans; Jakob P. Ley; Gerhard E. Krammer; Veronika Somoza

doi:10.1021/acs.jafc.8b01802

Identification of Bitter-Taste Intensity and Molecular Weight as Amino Acid Determinants for the Stimulating Mechanisms of Gastric Acid Secretion in Human Parietal Cells in Culture

Verena Stoeger, Kathrin I. Liszt, Barbara Lieder, Martin Wendelin, Muhammet Zopun, Joachim Hans, Jakob P. Ley, Gerhard E. Krammer, Veronika Somoza

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Secretion of gastric acid, aimed at preventing bacterial growth and aiding the digestion of foods in the stomach, is chiefly stimulated by dietary intake of protein and amino acids (AAs). However, AAs' key structural determinants responsible for their effects on mechanisms regulating gastric acid secretion (GAS) have not been identified yet. In this study, AAs have been tested in the parietal cell model HGT-1 on GAS and on mRNA expression of genes regulating GAS. AAs' taste intensities from 0 (not bitter at all) to 10 (very bitter) were assessed in a sensory study, in which ARG (l: 6.42 ± 0.41; d: 4.62 ± 0.59) and ILE (l: 4.21 ± 0.43; d: 2.28 ± 0.33) were identified as bitter-tasting candidates in both isomeric forms. Pearson correlation showed that GAS in HGT-1 cells is directly associated with the bitter taste quality (r: -0.654) in combination with the molecular weight of l-AA (r: -0.685).

Original language	English
Pages (from-to)	6762-6771
Number of pages	10
Journal	Journal of agricultural and food chemistry
Volume	66
Issue number	26
DOIs	https://doi.org/10.1021/acs.jafc.8b01802
State	Published - 5 Jul 2018
Externally published	Yes

Keywords

HGT-1
amino acids
bitter
gastric acid secretion
parietal cells

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10.1021/acs.jafc.8b01802

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Stoeger, V., Liszt, K. I., Lieder, B., Wendelin, M., Zopun, M., Hans, J., Ley, J. P., Krammer, G. E., & Somoza, V. (2018). Identification of Bitter-Taste Intensity and Molecular Weight as Amino Acid Determinants for the Stimulating Mechanisms of Gastric Acid Secretion in Human Parietal Cells in Culture. Journal of agricultural and food chemistry, 66(26), 6762-6771. https://doi.org/10.1021/acs.jafc.8b01802

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title = "Identification of Bitter-Taste Intensity and Molecular Weight as Amino Acid Determinants for the Stimulating Mechanisms of Gastric Acid Secretion in Human Parietal Cells in Culture",

abstract = "Secretion of gastric acid, aimed at preventing bacterial growth and aiding the digestion of foods in the stomach, is chiefly stimulated by dietary intake of protein and amino acids (AAs). However, AAs' key structural determinants responsible for their effects on mechanisms regulating gastric acid secretion (GAS) have not been identified yet. In this study, AAs have been tested in the parietal cell model HGT-1 on GAS and on mRNA expression of genes regulating GAS. AAs' taste intensities from 0 (not bitter at all) to 10 (very bitter) were assessed in a sensory study, in which ARG (l: 6.42 ± 0.41; d: 4.62 ± 0.59) and ILE (l: 4.21 ± 0.43; d: 2.28 ± 0.33) were identified as bitter-tasting candidates in both isomeric forms. Pearson correlation showed that GAS in HGT-1 cells is directly associated with the bitter taste quality (r: -0.654) in combination with the molecular weight of l-AA (r: -0.685).",

keywords = "HGT-1, amino acids, bitter, gastric acid secretion, parietal cells",

author = "Verena Stoeger and Liszt, {Kathrin I.} and Barbara Lieder and Martin Wendelin and Muhammet Zopun and Joachim Hans and Ley, {Jakob P.} and Krammer, {Gerhard E.} and Veronika Somoza",

note = "Publisher Copyright: {\textcopyright} 2018 American Chemical Society.",

year = "2018",

month = jul,

day = "5",

doi = "10.1021/acs.jafc.8b01802",

language = "English",

volume = "66",

pages = "6762--6771",

journal = "Journal of agricultural and food chemistry",

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Stoeger, V, Liszt, KI, Lieder, B, Wendelin, M, Zopun, M, Hans, J, Ley, JP, Krammer, GE & Somoza, V 2018, 'Identification of Bitter-Taste Intensity and Molecular Weight as Amino Acid Determinants for the Stimulating Mechanisms of Gastric Acid Secretion in Human Parietal Cells in Culture', Journal of agricultural and food chemistry, vol. 66, no. 26, pp. 6762-6771. https://doi.org/10.1021/acs.jafc.8b01802

Identification of Bitter-Taste Intensity and Molecular Weight as Amino Acid Determinants for the Stimulating Mechanisms of Gastric Acid Secretion in Human Parietal Cells in Culture. / Stoeger, Verena; Liszt, Kathrin I.; Lieder, Barbara et al.
In: Journal of agricultural and food chemistry, Vol. 66, No. 26, 05.07.2018, p. 6762-6771.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Identification of Bitter-Taste Intensity and Molecular Weight as Amino Acid Determinants for the Stimulating Mechanisms of Gastric Acid Secretion in Human Parietal Cells in Culture

AU - Stoeger, Verena

AU - Liszt, Kathrin I.

AU - Lieder, Barbara

AU - Wendelin, Martin

AU - Zopun, Muhammet

AU - Hans, Joachim

AU - Ley, Jakob P.

AU - Krammer, Gerhard E.

AU - Somoza, Veronika

PY - 2018/7/5

Y1 - 2018/7/5

N2 - Secretion of gastric acid, aimed at preventing bacterial growth and aiding the digestion of foods in the stomach, is chiefly stimulated by dietary intake of protein and amino acids (AAs). However, AAs' key structural determinants responsible for their effects on mechanisms regulating gastric acid secretion (GAS) have not been identified yet. In this study, AAs have been tested in the parietal cell model HGT-1 on GAS and on mRNA expression of genes regulating GAS. AAs' taste intensities from 0 (not bitter at all) to 10 (very bitter) were assessed in a sensory study, in which ARG (l: 6.42 ± 0.41; d: 4.62 ± 0.59) and ILE (l: 4.21 ± 0.43; d: 2.28 ± 0.33) were identified as bitter-tasting candidates in both isomeric forms. Pearson correlation showed that GAS in HGT-1 cells is directly associated with the bitter taste quality (r: -0.654) in combination with the molecular weight of l-AA (r: -0.685).

AB - Secretion of gastric acid, aimed at preventing bacterial growth and aiding the digestion of foods in the stomach, is chiefly stimulated by dietary intake of protein and amino acids (AAs). However, AAs' key structural determinants responsible for their effects on mechanisms regulating gastric acid secretion (GAS) have not been identified yet. In this study, AAs have been tested in the parietal cell model HGT-1 on GAS and on mRNA expression of genes regulating GAS. AAs' taste intensities from 0 (not bitter at all) to 10 (very bitter) were assessed in a sensory study, in which ARG (l: 6.42 ± 0.41; d: 4.62 ± 0.59) and ILE (l: 4.21 ± 0.43; d: 2.28 ± 0.33) were identified as bitter-tasting candidates in both isomeric forms. Pearson correlation showed that GAS in HGT-1 cells is directly associated with the bitter taste quality (r: -0.654) in combination with the molecular weight of l-AA (r: -0.685).

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KW - gastric acid secretion

KW - parietal cells

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AN - SCOPUS:85048367927

SN - 0021-8561

VL - 66

SP - 6762

EP - 6771

JO - Journal of agricultural and food chemistry

JF - Journal of agricultural and food chemistry

IS - 26

ER -

Identification of Bitter-Taste Intensity and Molecular Weight as Amino Acid Determinants for the Stimulating Mechanisms of Gastric Acid Secretion in Human Parietal Cells in Culture

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