Identification of Bacillus licheniformis by Colony Hybridization with 23S rRNA-targeted Oligonucleotide Probes

Ralf Tatzel, Wolfgang Ludwig, Karl Heinz Schleifer, Peter R. Wallnöfer

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

A rapid method for the identification of bacteria was developed which can be performed even in a routine laboratory. The sequence of 23S rRNA genes of Bacillus licheniformis was determined and two complementary oligonucleotide probes were synthesized. The specifity of the probes was checked by non-radioactive dot blot and colony hybridization experiments with Bacillus type strains typically occurring in food and with original isolates from milk and cream. One oligonucleotide probe hybridized to target nucleic acids from all Bacillus licheniformis strains tested, whereas the second probe was specific for only part of the strains studied. The probes can be used for the specific and fast detection and identification of Bacillus licheniformis.

Original languageEnglish
Pages (from-to)99-103
Number of pages5
JournalSystematic and Applied Microbiology
Volume17
Issue number1
DOIs
StatePublished - 1994
Externally publishedYes

Keywords

  • 23S rRNA Oligonucleotide probe
  • Bacillus licheniformis
  • Colony hybridization
  • Milk samples

Fingerprint

Dive into the research topics of 'Identification of Bacillus licheniformis by Colony Hybridization with 23S rRNA-targeted Oligonucleotide Probes'. Together they form a unique fingerprint.

Cite this