Identification of a gephyrin binding motif on the glycine receptor β subunit

Guido Meyer; Joachim Kirsch; Heinrich Betz; Dieter Langosch

doi:10.1016/0896-6273(95)90145-0

Identification of a gephyrin binding motif on the glycine receptor β subunit

Guido Meyer, Joachim Kirsch, Heinrich Betz, Dieter Langosch

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Abstract

The tubulin-binding protein gephyrin copurifies with the inhibitory glycine receptor (GIyR) and is essential for its postsynaptic localization. Here we have analyzed the interaction between the GIyR and recombinant gephyrin and identified a gephyrin binding site in the cytoplasmic loop between the third and fourth transmembrane segments of the β subunit. GIyR α subunits and GABA_A receptor proteins failed to bind recombinant gephyrin. However, insertion of an 18 residue segment of the GIyR β subunit into the GABA_A receptor β1 subunit conferred gephyrin binding both in an overlay assay and in transfected mammalian cells. These results indicate that β subunit expression is essential for the formation of a postsynaptic GIyR matrix.

Original language	English
Pages (from-to)	563-572
Number of pages	10
Journal	Neuron
Volume	15
Issue number	3
DOIs	https://doi.org/10.1016/0896-6273(95)90145-0
State	Published - Sep 1995
Externally published	Yes

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10.1016/0896-6273(95)90145-0

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title = "Identification of a gephyrin binding motif on the glycine receptor β subunit",

abstract = "The tubulin-binding protein gephyrin copurifies with the inhibitory glycine receptor (GIyR) and is essential for its postsynaptic localization. Here we have analyzed the interaction between the GIyR and recombinant gephyrin and identified a gephyrin binding site in the cytoplasmic loop between the third and fourth transmembrane segments of the β subunit. GIyR α subunits and GABAA receptor proteins failed to bind recombinant gephyrin. However, insertion of an 18 residue segment of the GIyR β subunit into the GABAA receptor β1 subunit conferred gephyrin binding both in an overlay assay and in transfected mammalian cells. These results indicate that β subunit expression is essential for the formation of a postsynaptic GIyR matrix.",

author = "Guido Meyer and Joachim Kirsch and Heinrich Betz and Dieter Langosch",

note = "Funding Information: We thank A. Herbold and J. Lorentz for expert technical assistance with mutagenesis and tissue culture, Dr. A. Stephenson for providing the Ro7/1986-1 -agarose, Dr. J. M. Fritsch for the GABAAR ~1 subunit-specific antibody, and Drs. P. Seeburg and H. LOddens for GABAAR subunit cDNAs. This work was supported by the Deutsche Forschun-gsgemeinschaft (SFB 169 and Schwerpunkt {"}Funktionelle Dom&nen{"}) and the Fends der Chemischen Industrie. J. K. and D. L. are supported by Heisenberg Fellowships of the Deutsche Forschungsgemeinschaft.",

year = "1995",

month = sep,

doi = "10.1016/0896-6273(95)90145-0",

language = "English",

volume = "15",

pages = "563--572",

journal = "Neuron",

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publisher = "Cell Press",

number = "3",

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T1 - Identification of a gephyrin binding motif on the glycine receptor β subunit

AU - Meyer, Guido

AU - Kirsch, Joachim

AU - Betz, Heinrich

AU - Langosch, Dieter

N1 - Funding Information: We thank A. Herbold and J. Lorentz for expert technical assistance with mutagenesis and tissue culture, Dr. A. Stephenson for providing the Ro7/1986-1 -agarose, Dr. J. M. Fritsch for the GABAAR ~1 subunit-specific antibody, and Drs. P. Seeburg and H. LOddens for GABAAR subunit cDNAs. This work was supported by the Deutsche Forschun-gsgemeinschaft (SFB 169 and Schwerpunkt "Funktionelle Dom&nen") and the Fends der Chemischen Industrie. J. K. and D. L. are supported by Heisenberg Fellowships of the Deutsche Forschungsgemeinschaft.

PY - 1995/9

Y1 - 1995/9

N2 - The tubulin-binding protein gephyrin copurifies with the inhibitory glycine receptor (GIyR) and is essential for its postsynaptic localization. Here we have analyzed the interaction between the GIyR and recombinant gephyrin and identified a gephyrin binding site in the cytoplasmic loop between the third and fourth transmembrane segments of the β subunit. GIyR α subunits and GABAA receptor proteins failed to bind recombinant gephyrin. However, insertion of an 18 residue segment of the GIyR β subunit into the GABAA receptor β1 subunit conferred gephyrin binding both in an overlay assay and in transfected mammalian cells. These results indicate that β subunit expression is essential for the formation of a postsynaptic GIyR matrix.

AB - The tubulin-binding protein gephyrin copurifies with the inhibitory glycine receptor (GIyR) and is essential for its postsynaptic localization. Here we have analyzed the interaction between the GIyR and recombinant gephyrin and identified a gephyrin binding site in the cytoplasmic loop between the third and fourth transmembrane segments of the β subunit. GIyR α subunits and GABAA receptor proteins failed to bind recombinant gephyrin. However, insertion of an 18 residue segment of the GIyR β subunit into the GABAA receptor β1 subunit conferred gephyrin binding both in an overlay assay and in transfected mammalian cells. These results indicate that β subunit expression is essential for the formation of a postsynaptic GIyR matrix.

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U2 - 10.1016/0896-6273(95)90145-0

DO - 10.1016/0896-6273(95)90145-0

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AN - SCOPUS:0029124881

SN - 0896-6273

VL - 15

SP - 563

EP - 572

JO - Neuron

JF - Neuron

IS - 3

ER -

Identification of a gephyrin binding motif on the glycine receptor β subunit

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