Identification of 1,2,4-trichlorobenzene-mineralizing bacteria and their function analysis

Fang Wang; U. Dorfler; M. Schmid; S. Grundmann; J. C. Munch; Xin Jiang; R. Schroll

Identification of 1,2,4-trichlorobenzene-mineralizing bacteria and their function analysis

Fang Wang
, U. Dorfler
, M. Schmid
, S. Grundmann
, J. C. Munch
, Xin Jiang
, R. Schroll

Chinese Academy of Sciences
Helmholtz Zentrum München German Research Center for Environmental Health

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Two strains, E3 and F2, capable to mineralize 1,2,4-trichlorobenzene (1,2,4-TCB) were isolated from a chlorinated benzenes contaminated soil using ¹⁴C-1,2,4-TCB as carbon source. They were identified by their 16S rDNA coding genes and fluorescence in situ hybridization (FISH) analysis as members of the genus Bordetella. A similarity of 100% were observed between strains E3 and F2 with their 16S rDNA sequences. They had the highest homology of 100% with Bordetella sp. QJ2-5 and the closest relation to described species, Bordetella petrii (GDH030510) with a similarity of 99.4%. Strains E3 and F2 could degrade about 90% of 1,2,4-TCB and mineralize 58% and 46% of 1,2,4-TCB to CO₂ within 30 days in mineral liquid cultures, respectively. Biomass was formed during the mineralization process.

Original language	English
Pages (from-to)	1082-1087
Number of pages	6
Journal	Huanjing Kexue/Environmental Science
Volume	28
Issue number	5
State	Published - May 2007
Externally published	Yes

Keywords

16S rDNA
Biodegradation
Bordetella sp.
C-labeled
Fluorescence in situ hybridization (FISH)
TCB

Cite this

@article{7e00f793bb9e4b2292566bf781f8ece0,

title = "Identification of 1,2,4-trichlorobenzene-mineralizing bacteria and their function analysis",

abstract = "Two strains, E3 and F2, capable to mineralize 1,2,4-trichlorobenzene (1,2,4-TCB) were isolated from a chlorinated benzenes contaminated soil using 14C-1,2,4-TCB as carbon source. They were identified by their 16S rDNA coding genes and fluorescence in situ hybridization (FISH) analysis as members of the genus Bordetella. A similarity of 100\% were observed between strains E3 and F2 with their 16S rDNA sequences. They had the highest homology of 100\% with Bordetella sp. QJ2-5 and the closest relation to described species, Bordetella petrii (GDH030510) with a similarity of 99.4\%. Strains E3 and F2 could degrade about 90\% of 1,2,4-TCB and mineralize 58\% and 46\% of 1,2,4-TCB to CO2 within 30 days in mineral liquid cultures, respectively. Biomass was formed during the mineralization process.",

keywords = "16S rDNA, Biodegradation, Bordetella sp., C-labeled, Fluorescence in situ hybridization (FISH), TCB",

author = "Fang Wang and U. Dorfler and M. Schmid and S. Grundmann and Munch, \{J. C.\} and Xin Jiang and R. Schroll",

year = "2007",

month = may,

language = "English",

volume = "28",

pages = "1082--1087",

journal = "Huanjing Kexue/Environmental Science",

issn = "0250-3301",

publisher = "Science Press",

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}

TY - JOUR

T1 - Identification of 1,2,4-trichlorobenzene-mineralizing bacteria and their function analysis

AU - Wang, Fang

AU - Dorfler, U.

AU - Schmid, M.

AU - Grundmann, S.

AU - Munch, J. C.

AU - Jiang, Xin

AU - Schroll, R.

PY - 2007/5

Y1 - 2007/5

N2 - Two strains, E3 and F2, capable to mineralize 1,2,4-trichlorobenzene (1,2,4-TCB) were isolated from a chlorinated benzenes contaminated soil using 14C-1,2,4-TCB as carbon source. They were identified by their 16S rDNA coding genes and fluorescence in situ hybridization (FISH) analysis as members of the genus Bordetella. A similarity of 100% were observed between strains E3 and F2 with their 16S rDNA sequences. They had the highest homology of 100% with Bordetella sp. QJ2-5 and the closest relation to described species, Bordetella petrii (GDH030510) with a similarity of 99.4%. Strains E3 and F2 could degrade about 90% of 1,2,4-TCB and mineralize 58% and 46% of 1,2,4-TCB to CO2 within 30 days in mineral liquid cultures, respectively. Biomass was formed during the mineralization process.

AB - Two strains, E3 and F2, capable to mineralize 1,2,4-trichlorobenzene (1,2,4-TCB) were isolated from a chlorinated benzenes contaminated soil using 14C-1,2,4-TCB as carbon source. They were identified by their 16S rDNA coding genes and fluorescence in situ hybridization (FISH) analysis as members of the genus Bordetella. A similarity of 100% were observed between strains E3 and F2 with their 16S rDNA sequences. They had the highest homology of 100% with Bordetella sp. QJ2-5 and the closest relation to described species, Bordetella petrii (GDH030510) with a similarity of 99.4%. Strains E3 and F2 could degrade about 90% of 1,2,4-TCB and mineralize 58% and 46% of 1,2,4-TCB to CO2 within 30 days in mineral liquid cultures, respectively. Biomass was formed during the mineralization process.

KW - 16S rDNA

KW - Biodegradation

KW - Bordetella sp.

KW - C-labeled

KW - Fluorescence in situ hybridization (FISH)

KW - TCB

UR - https://www.scopus.com/pages/publications/34249856135

M3 - Article

C2 - 17633183

AN - SCOPUS:34249856135

SN - 0250-3301

VL - 28

SP - 1082

EP - 1087

JO - Huanjing Kexue/Environmental Science

JF - Huanjing Kexue/Environmental Science

IS - 5

ER -

Identification of 1,2,4-trichlorobenzene-mineralizing bacteria and their function analysis

Abstract

Keywords

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