TY - JOUR
T1 - Identification and characterization of a mouse monoclonal antibody (M10) directed against canine (dog) CD8+ lymphocytes
AU - Voß, C.
AU - Kremmer, E.
AU - Hoffmann-Fezer, G.
AU - Schumm, M.
AU - Günther, W.
AU - Kolb, H. J.
AU - Thierfelder, S.
PY - 1993/10
Y1 - 1993/10
N2 - Monoclonal antibodies (mAb) were produced by immunizing BALB/c mice with non-adherent dog lymphocytes. M10 was specific for a subset of dog lymphocytes. M10 belonged to the IgG1 subclass and reacted with 26% of dog peripheral blood lymphocytes, 24% of spleen lymphocytes, 81% of thymus cells, 1.2% of bone marrow cells (5.8% of bone marrow lymphocytes) and 23% of PHA-stimulated lymphocytes. Immunohistology of snap-frozen thymus and spleen showed that the spleen B-cell area stained negative, whereas the spleen T-cell area and the thymus medulla exhibited positive reaction in 20-30%. The thymus cortex was strongly positive. M10 diminished cell lysis by 58% in cell mediated lysis assays (CML). Immunoblot assays revealed that M10 recognized an antigen with a molecular weight of 76 kD under non-reducing and 33 kD under reducing conditions. Finally, M10 bound to a canine CD8α transfected rat T-cell line (NB2). These findings characterize M10 as an antibody directed against the dog CD8 antigen.
AB - Monoclonal antibodies (mAb) were produced by immunizing BALB/c mice with non-adherent dog lymphocytes. M10 was specific for a subset of dog lymphocytes. M10 belonged to the IgG1 subclass and reacted with 26% of dog peripheral blood lymphocytes, 24% of spleen lymphocytes, 81% of thymus cells, 1.2% of bone marrow cells (5.8% of bone marrow lymphocytes) and 23% of PHA-stimulated lymphocytes. Immunohistology of snap-frozen thymus and spleen showed that the spleen B-cell area stained negative, whereas the spleen T-cell area and the thymus medulla exhibited positive reaction in 20-30%. The thymus cortex was strongly positive. M10 diminished cell lysis by 58% in cell mediated lysis assays (CML). Immunoblot assays revealed that M10 recognized an antigen with a molecular weight of 76 kD under non-reducing and 33 kD under reducing conditions. Finally, M10 bound to a canine CD8α transfected rat T-cell line (NB2). These findings characterize M10 as an antibody directed against the dog CD8 antigen.
UR - http://www.scopus.com/inward/record.url?scp=0027451697&partnerID=8YFLogxK
U2 - 10.1016/0165-2427(93)90090-Q
DO - 10.1016/0165-2427(93)90090-Q
M3 - Article
C2 - 8291208
AN - SCOPUS:0027451697
SN - 0165-2427
VL - 38
SP - 311
EP - 325
JO - Veterinary Immunology and Immunopathology
JF - Veterinary Immunology and Immunopathology
IS - 3-4
ER -