Hydroxylation of the B-Ring of Flavonoids in the 3′-and 5′-Position with Enzyme Extracts from Flowers of Verbena hybrida

G. Stotz, G. Forkmann

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

Enzyme preparations from flowers of Verbena hybrida do not only catalyse hydroxylation of the B-ring of flavanones and dihydroflavonols in the 3′-position but also in the 5′-position. Enzyme activity for 3′,5′-hydroxylation was found to be localized in the microsomal fraction and required NAD PH as cofactor. Evidence is provided that the formation of the 3′,4′,5′-hydroxylated flavanone (5,7,3′,4′,5′-pentahydroxyflavanone) and dihydroflavonol (dihydromyricetin), respectively, proceeds via the corresponding 3′,4′-hydroxylated compounds eriodictyol and dihydroquercetin, respectively, which are most probably formed by action of the same enzyme. Enzyme activity for 3′,5′-hydroxylation was found to be strictly correlated with the prescence of 3′,4′,5′-hydroxylated flavonoid compounds in the flowers.

Original languageEnglish
Pages (from-to)19-23
Number of pages5
JournalZeitschrift fur Naturforschung - Section C Journal of Biosciences
Volume37
Issue number1-2
DOIs
StatePublished - 1 Feb 1982
Externally publishedYes

Keywords

  • Anthocyanins
  • Biosynthesis
  • Flavonoid 3′,5′-Hydroxylation
  • Flavonoids
  • Verbena hybrida

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