Abstract
Innovative methods designed to recapitulate human organogenesis from pluripotent stem cells provide a means to explore human developmental biology. New technologies to sequence and analyze single-cell transcriptomes can deconstruct these ‘organoids’ into constituent parts, and reconstruct lineage trajectories during cell differentiation. In this Spotlight article we summarize the different approaches to performing single-cell transcriptomics on organoids, and discuss the opportunities and challenges of applying these techniques to generate organ-level, mechanistic models of human development and disease. Together, these technologies will move past characterization to the prediction of human developmental and disease-related phenomena.
Original language | English |
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Pages (from-to) | 1584-1587 |
Number of pages | 4 |
Journal | Development (Cambridge) |
Volume | 144 |
Issue number | 9 |
DOIs | |
State | Published - 1 May 2017 |
Keywords
- Human development
- Organogenesis
- Organoid
- Pluripotent stem cell
- Single-cell transcriptomics
- Transcriptome