Human mesenchymal stromal cells from adipose tissue of the neck

Florian Böhrnsen; Nicole Rotter; Ulrich Lindner; Markus Meier; Barbara Wollenberg; Jürgen Rohwedel; Jan Kramer

doi:10.1007/s00405-012-1943-5

Human mesenchymal stromal cells from adipose tissue of the neck

Florian Böhrnsen, Nicole Rotter, Ulrich Lindner, Markus Meier, Barbara Wollenberg, Jürgen Rohwedel, Jan Kramer

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Mesenchymal stromal cells (MSC) have been introduced into the Weld of tissue-engineered airway transplantation. Since patients with extensive tracheal defects often require an open tracheotomy, this study investigated if MSC could be obtained from the adipose tissue of the neck during this procedure. Cells were isolated by plastic adherence from the adipose tissue of 8 patients. Cell isolates were analyzed for (i) proliferation, (ii) the expression of CD marker molecules and (iii) multilineage differentiation. The isolated spindle-shaped cells showed a high proliferation capacity and the flow cytometric analysis revealed a distinct population meeting the criteria for MSC. Using classical MSC cultivation protocols the characterized cells showed adipogenic, chondrogenic and osteogenic differentiation for all analyzed cell isolates. This study was able to demonstrate that suYcient amounts of stem/progenitor cells can be easily isolated from adipose tissue of the neck obtained during open tracheotomy. These cells may be a source for future tracheal replacement therapies.

Original language	English
Pages (from-to)	2561-2570
Number of pages	10
Journal	European Archives of Oto-Rhino-Laryngology
Volume	269
Issue number	12
DOIs	https://doi.org/10.1007/s00405-012-1943-5
State	Published - Dec 2012
Externally published	Yes

Keywords

Adipose tissue
MSC
Mesenchymal stem cells
Mesenchymal stromal cells
Tissue engineering
Tracheal transplantation
Tracheotomy

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10.1007/s00405-012-1943-5

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title = "Human mesenchymal stromal cells from adipose tissue of the neck",

abstract = "Mesenchymal stromal cells (MSC) have been introduced into the Weld of tissue-engineered airway transplantation. Since patients with extensive tracheal defects often require an open tracheotomy, this study investigated if MSC could be obtained from the adipose tissue of the neck during this procedure. Cells were isolated by plastic adherence from the adipose tissue of 8 patients. Cell isolates were analyzed for (i) proliferation, (ii) the expression of CD marker molecules and (iii) multilineage differentiation. The isolated spindle-shaped cells showed a high proliferation capacity and the flow cytometric analysis revealed a distinct population meeting the criteria for MSC. Using classical MSC cultivation protocols the characterized cells showed adipogenic, chondrogenic and osteogenic differentiation for all analyzed cell isolates. This study was able to demonstrate that suYcient amounts of stem/progenitor cells can be easily isolated from adipose tissue of the neck obtained during open tracheotomy. These cells may be a source for future tracheal replacement therapies.",

keywords = "Adipose tissue, MSC, Mesenchymal stem cells, Mesenchymal stromal cells, Tissue engineering, Tracheal transplantation, Tracheotomy",

author = "Florian B{\"o}hrnsen and Nicole Rotter and Ulrich Lindner and Markus Meier and Barbara Wollenberg and J{\"u}rgen Rohwedel and Jan Kramer",

note = "Funding Information: The work was supported by the Medical Faculty of the University of L{\"u}beck (Program Regenerative Medicine and the Junior Research Cluster: Epithelial inflammation, L{\"u}beck, Germany) and Intermed Service GmbH & Co. KG (Geesthacht, Germany). The authors have no further interest in companies or other entities that have an interest in the information in the contribution. There is no conflict of interest pending. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. Funding Information: The work was supported by the Medical Faculty of the University of L{\"u}beck (Program Regenerative Medicine and the Junior Research Cluster: Epithelial inflammation, L{\"u}beck, Germany) and Intermed Service GmbH & Co. KG (Geesthacht, Germany). We are grateful to Barbara Andresen and Alexandra Tiedtke for their technical assistance. ",

year = "2012",

month = dec,

doi = "10.1007/s00405-012-1943-5",

language = "English",

volume = "269",

pages = "2561--2570",

journal = "European Archives of Oto-Rhino-Laryngology",

issn = "0937-4477",

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TY - JOUR

T1 - Human mesenchymal stromal cells from adipose tissue of the neck

AU - Böhrnsen, Florian

AU - Rotter, Nicole

AU - Lindner, Ulrich

AU - Meier, Markus

AU - Wollenberg, Barbara

AU - Rohwedel, Jürgen

AU - Kramer, Jan

N1 - Funding Information: The work was supported by the Medical Faculty of the University of Lübeck (Program Regenerative Medicine and the Junior Research Cluster: Epithelial inflammation, Lübeck, Germany) and Intermed Service GmbH & Co. KG (Geesthacht, Germany). The authors have no further interest in companies or other entities that have an interest in the information in the contribution. There is no conflict of interest pending. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. Funding Information: The work was supported by the Medical Faculty of the University of Lübeck (Program Regenerative Medicine and the Junior Research Cluster: Epithelial inflammation, Lübeck, Germany) and Intermed Service GmbH & Co. KG (Geesthacht, Germany). We are grateful to Barbara Andresen and Alexandra Tiedtke for their technical assistance.

PY - 2012/12

Y1 - 2012/12

N2 - Mesenchymal stromal cells (MSC) have been introduced into the Weld of tissue-engineered airway transplantation. Since patients with extensive tracheal defects often require an open tracheotomy, this study investigated if MSC could be obtained from the adipose tissue of the neck during this procedure. Cells were isolated by plastic adherence from the adipose tissue of 8 patients. Cell isolates were analyzed for (i) proliferation, (ii) the expression of CD marker molecules and (iii) multilineage differentiation. The isolated spindle-shaped cells showed a high proliferation capacity and the flow cytometric analysis revealed a distinct population meeting the criteria for MSC. Using classical MSC cultivation protocols the characterized cells showed adipogenic, chondrogenic and osteogenic differentiation for all analyzed cell isolates. This study was able to demonstrate that suYcient amounts of stem/progenitor cells can be easily isolated from adipose tissue of the neck obtained during open tracheotomy. These cells may be a source for future tracheal replacement therapies.

AB - Mesenchymal stromal cells (MSC) have been introduced into the Weld of tissue-engineered airway transplantation. Since patients with extensive tracheal defects often require an open tracheotomy, this study investigated if MSC could be obtained from the adipose tissue of the neck during this procedure. Cells were isolated by plastic adherence from the adipose tissue of 8 patients. Cell isolates were analyzed for (i) proliferation, (ii) the expression of CD marker molecules and (iii) multilineage differentiation. The isolated spindle-shaped cells showed a high proliferation capacity and the flow cytometric analysis revealed a distinct population meeting the criteria for MSC. Using classical MSC cultivation protocols the characterized cells showed adipogenic, chondrogenic and osteogenic differentiation for all analyzed cell isolates. This study was able to demonstrate that suYcient amounts of stem/progenitor cells can be easily isolated from adipose tissue of the neck obtained during open tracheotomy. These cells may be a source for future tracheal replacement therapies.

KW - Adipose tissue

KW - MSC

KW - Mesenchymal stem cells

KW - Mesenchymal stromal cells

KW - Tissue engineering

KW - Tracheal transplantation

KW - Tracheotomy

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U2 - 10.1007/s00405-012-1943-5

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AN - SCOPUS:84871300745

SN - 0937-4477

VL - 269

SP - 2561

EP - 2570

JO - European Archives of Oto-Rhino-Laryngology

JF - European Archives of Oto-Rhino-Laryngology

IS - 12

ER -

Human mesenchymal stromal cells from adipose tissue of the neck

Abstract

Keywords

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