Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts

Angelika E. Schníeke; Alexander J. Kind; William A. Ritchie; Karen Mycock; Angela R. Scott; Marjorie Ritchie; Ian Wilmut; Alan Colman; Keith H.S. Campbell

doi:10.1126/science.278.5346.2130

Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts

Angelika E. Schníeke, Alexander J. Kind, William A. Ritchie, Karen Mycock, Angela R. Scott, Marjorie Ritchie, Ian Wilmut, Alan Colman, Keith H.S. Campbell

Research output: Contribution to journal › Article › peer-review

749 Scopus citations

Abstract

Ovine primary fetal fibroblasts were cotransfected with a neomycin resistance marker gene (neo) and a human coagulation factor IX genomic construct designed for expression of the encoded protein in sheep milk. Two cloned transfectants and a population of neomycin (G418)-resistant cells were used as donors for nuclear transfer to enucleated oocytes. Six transgenic lambs were liveborn: Three produced from cloned cells contained factor IX and neo transgenes, whereas three produced from the uncloned population contained the marker gene only. Somatic cells can therefore be subjected to genetic manipulation in vitro and produce viable animals by nuclear transfer. Production of transgenic sheep by nuclear transfer requires fewer than half the animals needed for pronuclear microinjection.

Original language	English
Pages (from-to)	2130-2133
Number of pages	4
Journal	Science
Volume	278
Issue number	5346
DOIs	https://doi.org/10.1126/science.278.5346.2130
State	Published - 19 Dec 1997
Externally published	Yes

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title = "Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts",

abstract = "Ovine primary fetal fibroblasts were cotransfected with a neomycin resistance marker gene (neo) and a human coagulation factor IX genomic construct designed for expression of the encoded protein in sheep milk. Two cloned transfectants and a population of neomycin (G418)-resistant cells were used as donors for nuclear transfer to enucleated oocytes. Six transgenic lambs were liveborn: Three produced from cloned cells contained factor IX and neo transgenes, whereas three produced from the uncloned population contained the marker gene only. Somatic cells can therefore be subjected to genetic manipulation in vitro and produce viable animals by nuclear transfer. Production of transgenic sheep by nuclear transfer requires fewer than half the animals needed for pronuclear microinjection.",

author = "Schn{\'i}eke, {Angelika E.} and Kind, {Alexander J.} and Ritchie, {William A.} and Karen Mycock and Scott, {Angela R.} and Marjorie Ritchie and Ian Wilmut and Alan Colman and Campbell, {Keith H.S.}",

year = "1997",

month = dec,

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doi = "10.1126/science.278.5346.2130",

language = "English",

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TY - JOUR

T1 - Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts

AU - Schníeke, Angelika E.

AU - Kind, Alexander J.

AU - Ritchie, William A.

AU - Mycock, Karen

AU - Scott, Angela R.

AU - Ritchie, Marjorie

AU - Wilmut, Ian

AU - Colman, Alan

AU - Campbell, Keith H.S.

PY - 1997/12/19

Y1 - 1997/12/19

N2 - Ovine primary fetal fibroblasts were cotransfected with a neomycin resistance marker gene (neo) and a human coagulation factor IX genomic construct designed for expression of the encoded protein in sheep milk. Two cloned transfectants and a population of neomycin (G418)-resistant cells were used as donors for nuclear transfer to enucleated oocytes. Six transgenic lambs were liveborn: Three produced from cloned cells contained factor IX and neo transgenes, whereas three produced from the uncloned population contained the marker gene only. Somatic cells can therefore be subjected to genetic manipulation in vitro and produce viable animals by nuclear transfer. Production of transgenic sheep by nuclear transfer requires fewer than half the animals needed for pronuclear microinjection.

AB - Ovine primary fetal fibroblasts were cotransfected with a neomycin resistance marker gene (neo) and a human coagulation factor IX genomic construct designed for expression of the encoded protein in sheep milk. Two cloned transfectants and a population of neomycin (G418)-resistant cells were used as donors for nuclear transfer to enucleated oocytes. Six transgenic lambs were liveborn: Three produced from cloned cells contained factor IX and neo transgenes, whereas three produced from the uncloned population contained the marker gene only. Somatic cells can therefore be subjected to genetic manipulation in vitro and produce viable animals by nuclear transfer. Production of transgenic sheep by nuclear transfer requires fewer than half the animals needed for pronuclear microinjection.

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SN - 0036-8075

VL - 278

SP - 2130

EP - 2133

JO - Science

JF - Science

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ER -

Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts

Abstract

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