@article{542f65a181414388aa2980246c4e3213,
title = "Hsp12 Is an Intrinsically Unstructured Stress Protein that Folds upon Membrane Association and Modulates Membrane Function",
abstract = "Hsp12 of S. cerevisiae is upregulated several 100-fold in response to stress. Our phenotypic analysis showed that this protein is important for survival of a variety of stress conditions, including high temperature. In the absence of Hsp12, we observed changes in cell morphology under stress conditions. Surprisingly, in the cell, Hsp12 exists both as a soluble cytosolic protein and associated to the plasma membrane. The in vitro analysis revealed that Hsp12, unlike all other Hsps studied so far, is completely unfolded; however, in the presence of certain lipids, it adopts a helical structure. The presence of Hsp12 does not alter the overall lipid composition of the plasma membrane but increases membrane stability.",
keywords = "Cellbio, Proteins",
author = "Sylvia Welker and Birgit Rudolph and Elke Frenzel and Franz Hagn and Gerhard Liebisch and Gerd Schmitz and Johannes Scheuring and Andreas Kerth and Alfred Blume and Sevil Weinkauf and Martin Haslbeck and Horst Kessler and Johannes Buchner",
note = "Funding Information: This work was supported by grants from the Deutsche Forschungsgemeinschaft and the Fonds der Chemischen Industrie to J.B., M.H., and H.K. F.H. is supported by the CompInt project of the Elitenetzwerk Bayern. We thank J. Seelig (Biozentrum, Uni Basel) for expert advice on the analysis of protein-lipid interactions by calorimetry, Bernd Reif and Rasmus Linser (Leibniz-Institut f{\"u}r Molekulare Pharmakologie [FMP] Berlin) for solid-state NMR measurements, and Tobias Madl (Technische Universit{\"a}t M{\"u}nchen) for providing the paramagnetic agent and for fitting PRE waves. Furthermore, we thank Bettina Richter and Francis Adigbli for assistance with electron microscopy, Helmut Krause for mass spectrometry analysis, Ralf Erdmann and Sabrina Mindthoff for help with the organelle fractionation, Alexander Bepperling for analytical ultracentrifugation, and Elke Deuerling and Yulia Ilina for assistance with flow cytometry. ",
year = "2010",
month = aug,
doi = "10.1016/j.molcel.2010.08.001",
language = "English",
volume = "39",
pages = "507--520",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "4",
}