Hole burning spectroscopy and physics of proteins

Josef Friedrich

doi:10.1016/0076-6879(95)46012-8

Hole burning spectroscopy and physics of proteins

Josef Friedrich

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Abstract

This chapter focuses on hole burning spectroscopy and physics of proteins. This chapter deals with the general aspects of the technique and the physics involved. This chapter also highlights the specific contributions from hole burning experiments to the understanding of photosynthesis. This chapter also discusses on the power of the hole burning technique in investigating disorder phenomena in proteins. By comparing proteins with crystals and glasses, this chapter shall try to characterize the specific features of the protein state of matter. This chapter presents the conclusion that even if such pronounced optical changes as the shift of a peak on the order of the inhomogeneous width are induced; the associated average structural changes are just at the resolution limit of X-rays as obtained from an analysis of the associated Debye-Waller Factors.

Original language	English
Pages (from-to)	226-259
Number of pages	34
Journal	Methods in Enzymology
Volume	246
Issue number	C
DOIs	https://doi.org/10.1016/0076-6879(95)46012-8
State	Published - 1 Jan 1995

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title = "Hole burning spectroscopy and physics of proteins",

abstract = "This chapter focuses on hole burning spectroscopy and physics of proteins. This chapter deals with the general aspects of the technique and the physics involved. This chapter also highlights the specific contributions from hole burning experiments to the understanding of photosynthesis. This chapter also discusses on the power of the hole burning technique in investigating disorder phenomena in proteins. By comparing proteins with crystals and glasses, this chapter shall try to characterize the specific features of the protein state of matter. This chapter presents the conclusion that even if such pronounced optical changes as the shift of a peak on the order of the inhomogeneous width are induced; the associated average structural changes are just at the resolution limit of X-rays as obtained from an analysis of the associated Debye-Waller Factors.",

author = "Josef Friedrich",

note = "Funding Information: The authors thank Mrs. M. L. Groot for providing Fig. 2, Mr. L. M. P. Beekman for providing Fig. 4, Dr. S. Lin for providing Fig, 5, and Dr. C. Kirmaier for providing Fig. 6. This work was supported by the Dutch Foundation for Biophysics (SvB), financed by the Netherlands Organization for Scientific Research (NWO). Funding Information: I thank J. Fidy, H. Frauenfelder,J . Gafert~F . Parak, and H. Scheerf or manys timulating discussions. I thank Prof. G. J. Small for sending me his publications on photosynthesis research. Financial support came from the DFG (Fr 456/17-1, SFB 213, GraduiertenkoUeg {"}Nichtlineare Dynamik{"}), the Fonds der Chemischen Industrie, and the WTZ program with Hungary.",

year = "1995",

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doi = "10.1016/0076-6879(95)46012-8",

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N1 - Funding Information: The authors thank Mrs. M. L. Groot for providing Fig. 2, Mr. L. M. P. Beekman for providing Fig. 4, Dr. S. Lin for providing Fig, 5, and Dr. C. Kirmaier for providing Fig. 6. This work was supported by the Dutch Foundation for Biophysics (SvB), financed by the Netherlands Organization for Scientific Research (NWO). Funding Information: I thank J. Fidy, H. Frauenfelder,J . Gafert~F . Parak, and H. Scheerf or manys timulating discussions. I thank Prof. G. J. Small for sending me his publications on photosynthesis research. Financial support came from the DFG (Fr 456/17-1, SFB 213, GraduiertenkoUeg "Nichtlineare Dynamik"), the Fonds der Chemischen Industrie, and the WTZ program with Hungary.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - This chapter focuses on hole burning spectroscopy and physics of proteins. This chapter deals with the general aspects of the technique and the physics involved. This chapter also highlights the specific contributions from hole burning experiments to the understanding of photosynthesis. This chapter also discusses on the power of the hole burning technique in investigating disorder phenomena in proteins. By comparing proteins with crystals and glasses, this chapter shall try to characterize the specific features of the protein state of matter. This chapter presents the conclusion that even if such pronounced optical changes as the shift of a peak on the order of the inhomogeneous width are induced; the associated average structural changes are just at the resolution limit of X-rays as obtained from an analysis of the associated Debye-Waller Factors.

AB - This chapter focuses on hole burning spectroscopy and physics of proteins. This chapter deals with the general aspects of the technique and the physics involved. This chapter also highlights the specific contributions from hole burning experiments to the understanding of photosynthesis. This chapter also discusses on the power of the hole burning technique in investigating disorder phenomena in proteins. By comparing proteins with crystals and glasses, this chapter shall try to characterize the specific features of the protein state of matter. This chapter presents the conclusion that even if such pronounced optical changes as the shift of a peak on the order of the inhomogeneous width are induced; the associated average structural changes are just at the resolution limit of X-rays as obtained from an analysis of the associated Debye-Waller Factors.

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Hole burning spectroscopy and physics of proteins

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