HLA class II mRNA accumulation by activated human T cells following growth in conditioned medium

B. Gansbacher, K. S. Zier

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The expression of HLA Class II β genes by seven CD4- or CD8-positive T cell clones grown in conditioned medium was examined on the mRNA and cell surface levels. Monoclonal antibodies directed against Class II glycoproteins demonstrated the presence of these molecules on the cell surface. In addition, Northern blot analysis demonstrated that cDNA probes for the DR, DP, and DQ β genes hybridized to mRNA isolated from each clone. Because we were interested in differences in expression of relative levels of DR, DQ and DP, mRNA isolated from the clones was quantified by means of densitometry. Or results demonstrated that different ratios of DR, DQ and DP β chain messages were expressed by the clones. Subsequent studies established the kinetics of the accumulation of DR β mRNA messages by T cells during culture and showed that it could be regulated by factors contained with conditioned medium. Our results confirm that the accumulation of Class II mRNA following growth of T cells in conditioned medium appears to be a general feature.

Original languageEnglish
Pages (from-to)440-445
Number of pages6
JournalClinical and Experimental Immunology
Volume76
Issue number3
StatePublished - 1989
Externally publishedYes

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