Highly parallel affinity sensor for the detection of environmental contaminants in water

Michael G. Weller; Andreas J. Schuetz; Michael Winklmair; Reinhard Niessner

doi:10.1016/S0003-2670(98)00855-1

Highly parallel affinity sensor for the detection of environmental contaminants in water

Michael G. Weller, Andreas J. Schuetz, Michael Winklmair, Reinhard Niessner

Natural Sciences

Technical University of Munich

Research output: Contribution to journal › Article › peer-review

65 Scopus citations

Abstract

A parallel affinity sensor array (PASA) was implemented based on chemiluminescence labels (peroxidase/luminol) and CCD detection. Reagents like antibodies or haptens have been immobilized on a glass slide, which forms a biochip with an active area of about 1.8cm². The spot density on the biochip was up to 9 spots per mm², which corresponds to an array of 1600 spots. Direct and indirect immunoassay formats have been tested successfully. Detection limits down to 20ngl^-1 terbutylazine could be reached. The regeneration of the indirect assay chips could be performed more than 100 times. To improve the regenerability of direct assays an enzyme tracer with a cleavable spacer was introduced. The potential of the PASA for analyte identification by pattern recognition and subsequent analyte quantification could be shown. The application of the system is discussed for environmental, pharmaceutical, and other analytical fields. Copyright (C) 1999 Elsevier Science B.V.

Original language	English
Pages (from-to)	29-41
Number of pages	13
Journal	Analytica Chimica Acta
Volume	393
Issue number	1-3
DOIs	https://doi.org/10.1016/S0003-2670(98)00855-1
State	Published - 30 Jun 1999

Keywords

2,4-D
Array
Immunosensor
Multianalyte detection
Regeneration
Triazine herbicides
Trinitrotoluene

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10.1016/S0003-2670(98)00855-1

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title = "Highly parallel affinity sensor for the detection of environmental contaminants in water",

abstract = "A parallel affinity sensor array (PASA) was implemented based on chemiluminescence labels (peroxidase/luminol) and CCD detection. Reagents like antibodies or haptens have been immobilized on a glass slide, which forms a biochip with an active area of about 1.8cm2. The spot density on the biochip was up to 9 spots per mm2, which corresponds to an array of 1600 spots. Direct and indirect immunoassay formats have been tested successfully. Detection limits down to 20ngl-1 terbutylazine could be reached. The regeneration of the indirect assay chips could be performed more than 100 times. To improve the regenerability of direct assays an enzyme tracer with a cleavable spacer was introduced. The potential of the PASA for analyte identification by pattern recognition and subsequent analyte quantification could be shown. The application of the system is discussed for environmental, pharmaceutical, and other analytical fields. Copyright (C) 1999 Elsevier Science B.V.",

keywords = "2,4-D, Array, Immunosensor, Multianalyte detection, Regeneration, Triazine herbicides, Trinitrotoluene",

author = "Weller, \{Michael G.\} and Schuetz, \{Andreas J.\} and Michael Winklmair and Reinhard Niessner",

note = "Funding Information: The financial support of the BMBF (project 02-WU9573/3) is gratefully acknowledged. We are indebted to Dr. Karu, University of California, Dr. Schlaeppi, Novartis, Basle, Strategic Diagnostics, Newark, NJ and Dr. Fr{\'a}nek, Veterinary Research Institute, Brno, Czech Republic, for providing us with the monoclonal antibodies AM7B2, 4063-21-1, TNT A/1.1.1 and E2/G2, respectively.",

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AU - Weller, Michael G.

AU - Schuetz, Andreas J.

AU - Winklmair, Michael

AU - Niessner, Reinhard

N1 - Funding Information: The financial support of the BMBF (project 02-WU9573/3) is gratefully acknowledged. We are indebted to Dr. Karu, University of California, Dr. Schlaeppi, Novartis, Basle, Strategic Diagnostics, Newark, NJ and Dr. Fránek, Veterinary Research Institute, Brno, Czech Republic, for providing us with the monoclonal antibodies AM7B2, 4063-21-1, TNT A/1.1.1 and E2/G2, respectively.

PY - 1999/6/30

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N2 - A parallel affinity sensor array (PASA) was implemented based on chemiluminescence labels (peroxidase/luminol) and CCD detection. Reagents like antibodies or haptens have been immobilized on a glass slide, which forms a biochip with an active area of about 1.8cm2. The spot density on the biochip was up to 9 spots per mm2, which corresponds to an array of 1600 spots. Direct and indirect immunoassay formats have been tested successfully. Detection limits down to 20ngl-1 terbutylazine could be reached. The regeneration of the indirect assay chips could be performed more than 100 times. To improve the regenerability of direct assays an enzyme tracer with a cleavable spacer was introduced. The potential of the PASA for analyte identification by pattern recognition and subsequent analyte quantification could be shown. The application of the system is discussed for environmental, pharmaceutical, and other analytical fields. Copyright (C) 1999 Elsevier Science B.V.

AB - A parallel affinity sensor array (PASA) was implemented based on chemiluminescence labels (peroxidase/luminol) and CCD detection. Reagents like antibodies or haptens have been immobilized on a glass slide, which forms a biochip with an active area of about 1.8cm2. The spot density on the biochip was up to 9 spots per mm2, which corresponds to an array of 1600 spots. Direct and indirect immunoassay formats have been tested successfully. Detection limits down to 20ngl-1 terbutylazine could be reached. The regeneration of the indirect assay chips could be performed more than 100 times. To improve the regenerability of direct assays an enzyme tracer with a cleavable spacer was introduced. The potential of the PASA for analyte identification by pattern recognition and subsequent analyte quantification could be shown. The application of the system is discussed for environmental, pharmaceutical, and other analytical fields. Copyright (C) 1999 Elsevier Science B.V.

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KW - Trinitrotoluene

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Highly parallel affinity sensor for the detection of environmental contaminants in water

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Keywords

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