High-throughput single-cell transcriptomics on organoids

Agnieska Brazovskaja, Barbara Treutlein, J. Gray Camp

Research output: Contribution to journalReview articlepeer-review

57 Scopus citations

Abstract

Three-dimensional (3D) tissues grown in culture from human stem cells offer the incredible opportunity to analyze and manipulate human development, and to generate patient-specific models of disease. Methods to sequence DNA and RNA in single cells are being used to analyze these so-called ‘organoid’ systems in high-resolution. Single-cell transcriptomics has been used to quantitate the similarity of organoid cells to primary tissue counterparts in the brain, intestine, liver, and kidney, as well as identify cell-specific responses to environmental variables and disease conditions. The merging of these two technologies, single-cell genomics and organoids, will have profound impact on personalized medicine in the near future.

Original languageEnglish
Pages (from-to)167-171
Number of pages5
JournalCurrent Opinion in Biotechnology
Volume55
DOIs
StatePublished - Feb 2019

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