TY - JOUR
T1 - High-throughput single-cell transcriptomics on organoids
AU - Brazovskaja, Agnieska
AU - Treutlein, Barbara
AU - Camp, J. Gray
N1 - Publisher Copyright:
© 2018
PY - 2019/2
Y1 - 2019/2
N2 - Three-dimensional (3D) tissues grown in culture from human stem cells offer the incredible opportunity to analyze and manipulate human development, and to generate patient-specific models of disease. Methods to sequence DNA and RNA in single cells are being used to analyze these so-called ‘organoid’ systems in high-resolution. Single-cell transcriptomics has been used to quantitate the similarity of organoid cells to primary tissue counterparts in the brain, intestine, liver, and kidney, as well as identify cell-specific responses to environmental variables and disease conditions. The merging of these two technologies, single-cell genomics and organoids, will have profound impact on personalized medicine in the near future.
AB - Three-dimensional (3D) tissues grown in culture from human stem cells offer the incredible opportunity to analyze and manipulate human development, and to generate patient-specific models of disease. Methods to sequence DNA and RNA in single cells are being used to analyze these so-called ‘organoid’ systems in high-resolution. Single-cell transcriptomics has been used to quantitate the similarity of organoid cells to primary tissue counterparts in the brain, intestine, liver, and kidney, as well as identify cell-specific responses to environmental variables and disease conditions. The merging of these two technologies, single-cell genomics and organoids, will have profound impact on personalized medicine in the near future.
UR - http://www.scopus.com/inward/record.url?scp=85057771922&partnerID=8YFLogxK
U2 - 10.1016/j.copbio.2018.11.002
DO - 10.1016/j.copbio.2018.11.002
M3 - Review article
C2 - 30504008
AN - SCOPUS:85057771922
SN - 0958-1669
VL - 55
SP - 167
EP - 171
JO - Current Opinion in Biotechnology
JF - Current Opinion in Biotechnology
ER -