TY - JOUR
T1 - High-throughput phenotypic assessment of cardiac physiology in four commonly used inbred mouse strains
AU - Moreth, Kristin
AU - Fischer, Ralf
AU - Fuchs, Helmut
AU - Gailus-Durner, Valérie
AU - Wurst, Wolfgang
AU - Katus, Hugo A.
AU - Bekeredjian, Raffi
AU - Hrabě de Angelis, Martin
N1 - Funding Information:
Acknowledgments We thank anna nießer for data collection and Kerstin Scheffel for proofreading the manuscript. The authors are grateful to the reviewers whose comments and suggestions significantly improved both clarity and precision of the paper. This study was supported by grants from 01KX1012 Infrafron-tier BMBF and Z56010015100 DZHK (Deutsches Zentrum für Herz-Kreislauf-Forschung).
PY - 2014/8
Y1 - 2014/8
N2 - Mice with genetic alterations are used in heart research as model systems of human diseases. In the last decade there was a marked increase in the recognition of genetic diversity within inbred mouse strains. Increasing numbers of inbred mouse strains and substrains and analytical variation of cardiac phenotyping methods require reproducible, high-throughput methods to standardize murine cardiovascular physiology. We describe methods for non-invasive, reliable, easy and fast to perform echocardiography and electrocardiography on awake mice. This method can be used for primary screening of the murine cardiovascular system in large-scale analysis. We provide insights into the physiological divergence of C57BL/6N, C57BL/6J, C3HeB/FeJ and 129P2/OlaHsd mouse hearts and define the expected normal values. Our report highlights that compared to the other three strains tested C57BL/6N hearts reveal features of heart failure such as hypertrophy and reduced contractile function. We found several features of the mouse ECG to be under genetic control and obtained several strain-specific differences in cardiac structure and function.
AB - Mice with genetic alterations are used in heart research as model systems of human diseases. In the last decade there was a marked increase in the recognition of genetic diversity within inbred mouse strains. Increasing numbers of inbred mouse strains and substrains and analytical variation of cardiac phenotyping methods require reproducible, high-throughput methods to standardize murine cardiovascular physiology. We describe methods for non-invasive, reliable, easy and fast to perform echocardiography and electrocardiography on awake mice. This method can be used for primary screening of the murine cardiovascular system in large-scale analysis. We provide insights into the physiological divergence of C57BL/6N, C57BL/6J, C3HeB/FeJ and 129P2/OlaHsd mouse hearts and define the expected normal values. Our report highlights that compared to the other three strains tested C57BL/6N hearts reveal features of heart failure such as hypertrophy and reduced contractile function. We found several features of the mouse ECG to be under genetic control and obtained several strain-specific differences in cardiac structure and function.
KW - C57BL/6
KW - Echocardiography
KW - Electrocardiography
KW - Heart
KW - Screening
UR - http://www.scopus.com/inward/record.url?scp=84905035248&partnerID=8YFLogxK
U2 - 10.1007/s00360-014-0830-3
DO - 10.1007/s00360-014-0830-3
M3 - Article
C2 - 24788387
AN - SCOPUS:84905035248
SN - 0174-1578
VL - 184
SP - 763
EP - 775
JO - Journal of Comparative Physiology - B Biochemical, Systemic, and Environmental Physiology
JF - Journal of Comparative Physiology - B Biochemical, Systemic, and Environmental Physiology
IS - 6
ER -