High-speed genotyping of CYP1A2*1F mutation with fluorescent hybridization probes using the LightCycler™

Johannes Popp, Barbara Messner, Werner Steimer

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The polymorphism CYP1A2*1F of the cytochrome 1A2 gene affects the inducibility of this cytochrome, which is involved in the metabolism of many commonly used drugs. We developed a rapid-cycle polymerase chain reaction (PCR) and fluorescent probe melting curve analysis on the LightCycler™ instrument for identification of this mutation. This new method was confirmed by genotyping 101 samples in parallel to restriction enzyme digestion. The 100% concordance of both methods showed the reliability of our high-speed genotyping assay, which is suitable for large epidemiological studies or routine clinical use.

Original languageEnglish
Pages (from-to)643-646
Number of pages4
JournalPharmacogenomics
Volume4
Issue number5
DOIs
StatePublished - Sep 2003

Keywords

  • CYP1A2
  • CYP1A2*1F
  • Genotyping
  • Melting curve analysis
  • Polymorphisms
  • Psychoactive drugs
  • Real time PCR

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