High resolution 1H detected 1H,13C correlation spectra in MAS solid-state NMR using deuterated proteins with selective1H,2H isotopic labeling of methyl groups

Vipin Agarwal, Anne Diehl, Nikolai Skrynnikov, Bernd Reif

Research output: Contribution to journalArticlepeer-review

105 Scopus citations

Abstract

MAS solid-state NMR experiments applied to biological solids are still hampered by low sensitivity and resolution. In this work, we employ a deuteration scheme in which individual methyl groups are selectively protonated. This labeling scheme allows the acquisition of proton carbon correlation spectra with a resolution comparable to that in solution-state NMR experiments. We observe an increase in resolution by a factor of 10-15 compared to standard heteronuclear correlation experiments using PMLG for 1H,1H dipolar decoupling in the indirect dimension. At the same time, the full sensitivity of the proton-based experiment is retained. In comparison to the heteronuclear detected version of the experiment, a gain in sensitivity of a factor of approximately 4.7 is achieved.

Original languageEnglish
Pages (from-to)12620-12621
Number of pages2
JournalJournal of the American Chemical Society
Volume128
Issue number39
DOIs
StatePublished - 4 Oct 2006
Externally publishedYes

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