High-resolution epi-illumination raster-scan optoacoustic mesoscopy for imaging of model organisms and microvessels

Murad Omar, Dominik Soliman, Jérôme Gateau, Vasilis Ntziachristos

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We have developed an epi-illumination raster-scan optoacoustic mesoscopy system (RSOM), the new system is capable of imaging model organisms, and vasculature. The newly developed system is based on a custom designed; spherically focused detector with a Characterization of the system shows an isotropic lateral resolution of 18 μm, and an axial resolution of 4 μm. The scan times are on the order of 8 minutes for a field of view of 10×10 mm2. The achieved resolution is slightly degraded up to a depth of 5 mm. After characterizing the system we showcase it's performance on a zebrafish ex vivo, and an excised mouse ear. Additionally, to improve the visibility of small structures we have reconstructed the high frequencies, and the low frequencies separately, and at the end overplayed the two reconstructions using different colors, this way the high frequencies are not masked by the low frequencies which have a higher signal to noise ratio.

Original languageEnglish
Title of host publicationEuropean Conference on Biomedical Optics, ECBO 2015
PublisherOptica Publishing Group (formerly OSA)
ISBN (Print)9781628417012
DOIs
StatePublished - 2015
Externally publishedYes
EventEuropean Conference on Biomedical Optics, ECBO 2015 - Munich, Germany
Duration: 21 Jun 201525 Jun 2015

Publication series

NameOptics InfoBase Conference Papers
ISSN (Electronic)2162-2701

Conference

ConferenceEuropean Conference on Biomedical Optics, ECBO 2015
Country/TerritoryGermany
CityMunich
Period21/06/1525/06/15

Keywords

  • Frequency analysis
  • Mesoscopy
  • Microcirculation
  • Optoacoustic
  • Photoacoustic
  • Skin

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