TY - JOUR
T1 - High performance liquid chromatography for the separation of histamine, its precursor, and metabolites
T2 - Application to biological samples
AU - Hermann, K.
AU - Frank, G.
AU - Ring, J.
N1 - Funding Information:
Acknowledgments The authors wsh to thank Mrs J Crosch and P Decker-Hermann for their excellent technical assistance H-RIA-Kits were generously provided by Dr B Manz, IBL, Hamburg, FRG The editorial help of Mrs P Decker-Hermann is gratefully acknowledged
PY - 1995/1/1
Y1 - 1995/1/1
N2 - Histamine was separated from its precursor L-histidine and its metabolites 1-metbylhista-mine and methylimidazole acetic acid on a TSK SP-5 PW cation exchange column and gradient elution. A baseline separation of histamine, 1-methylhistamine and methylimidazole acetic acid was also achieved under isocratic elution conditions on a reversed phase Cg column with a mobile phase of 15 % methanol and 85 % of an aqueous solution of 0.05 M NaH2PC>4 pH 3.1 which contained 0.5 mM EDTA-Na4 and 0.005 M octan-1-sulfonic acid sodium salt as an ion paring reagent. The most sensitive UV detection of histamine and 1-methylhistamine with the highest detector signal was obtained at a wavelength of 210 nm. In the absence of the ion pairing reagent and the organic modifier methanol, histamine and 1-methylhistamine were not retained on a reversed phase Cjg, Cg or C4 column. Octadecasilyl-silica cartridges were used to purify histamine from other constituents present in human urine and a commercially available heparin formulation. The analytical recovery of ^H-labeled histamine after the purification on octadecasilyl-silica cartridges was 95.16 ± 0.92 % (mean ± SEM, n=22). The concentration of the histamine-like material in urine samples from healthy volunteers was 46.28 ± 15.42 ug/24h (mean ± SEM; n=7). In the heparin fonnulation the histamine concentrations were 81.93 ng/ml before and 279.24 ng/ml after the purification on octadecasilyl-silca cartridges. The histamine-immunoreactive material in urine samples could be characterized on a TSK SP-5 PW cation exchange column as 19.69 • 7.86 % histamine and 46.74 ± 12.89 % 1-methylhistamine (mean ± SEM, n-7). Rechromatography of the 1-methylhistamin.e peak from the ion exchange column on the reversed phase Cg column disclosed a substance of unknown nature with a different retention time than 1-methylhistamine. Histamine purified from heparin eluted from the ion exchange column as a single peak with the same retention time as the histamine standard.
AB - Histamine was separated from its precursor L-histidine and its metabolites 1-metbylhista-mine and methylimidazole acetic acid on a TSK SP-5 PW cation exchange column and gradient elution. A baseline separation of histamine, 1-methylhistamine and methylimidazole acetic acid was also achieved under isocratic elution conditions on a reversed phase Cg column with a mobile phase of 15 % methanol and 85 % of an aqueous solution of 0.05 M NaH2PC>4 pH 3.1 which contained 0.5 mM EDTA-Na4 and 0.005 M octan-1-sulfonic acid sodium salt as an ion paring reagent. The most sensitive UV detection of histamine and 1-methylhistamine with the highest detector signal was obtained at a wavelength of 210 nm. In the absence of the ion pairing reagent and the organic modifier methanol, histamine and 1-methylhistamine were not retained on a reversed phase Cjg, Cg or C4 column. Octadecasilyl-silica cartridges were used to purify histamine from other constituents present in human urine and a commercially available heparin formulation. The analytical recovery of ^H-labeled histamine after the purification on octadecasilyl-silica cartridges was 95.16 ± 0.92 % (mean ± SEM, n=22). The concentration of the histamine-like material in urine samples from healthy volunteers was 46.28 ± 15.42 ug/24h (mean ± SEM; n=7). In the heparin fonnulation the histamine concentrations were 81.93 ng/ml before and 279.24 ng/ml after the purification on octadecasilyl-silca cartridges. The histamine-immunoreactive material in urine samples could be characterized on a TSK SP-5 PW cation exchange column as 19.69 • 7.86 % histamine and 46.74 ± 12.89 % 1-methylhistamine (mean ± SEM, n-7). Rechromatography of the 1-methylhistamin.e peak from the ion exchange column on the reversed phase Cg column disclosed a substance of unknown nature with a different retention time than 1-methylhistamine. Histamine purified from heparin eluted from the ion exchange column as a single peak with the same retention time as the histamine standard.
UR - http://www.scopus.com/inward/record.url?scp=0028889040&partnerID=8YFLogxK
U2 - 10.1080/10826079508009231
DO - 10.1080/10826079508009231
M3 - Article
AN - SCOPUS:0028889040
SN - 0148-3919
VL - 18
SP - 189
EP - 204
JO - Journal of Liquid Chromatography
JF - Journal of Liquid Chromatography
IS - 1
ER -