TY - JOUR
T1 - High Fat Diet Accelerates Pathogenesis of Murine Crohn's Disease-Like Ileitis Independently of Obesity
AU - Gruber, Lisa
AU - Kisling, Sigrid
AU - Lichti, Pia
AU - Martin, François Pierre
AU - May, Stephanie
AU - Klingenspor, Martin
AU - Lichtenegger, Martina
AU - Rychlik, Michael
AU - Haller, Dirk
N1 - Funding Information:
The authors are particularly grateful to Dr. George Kollias at the Biomedical Sciences Research Center “Alexander Fleming” (Vari, Greece) for providing the TNF mice. We thank Jürgen Danier and staff from the BIOANALYTIK Weihenstephan (ZIEL, Technische Universität München) for providing analytical data of the diets used. We thank Dr. Jalil Benyacoub, Dr. Nabil Bosco, Dr. Viral Brahmbhatt, Dr. Serge Rezzi, Dr. Sebastiano Collino and Dr. Ivan Montoliu at Nestlé Research Center (Lausanne, Switzerland) for scientific discussion and support for metabolomics analysis. Lisa Gruber gratefully acknowledges the support by the Faculty Graduate Center of TUM Graduate School at Technische Universität München, Germany. ΔARE/WT
PY - 2013/8/16
Y1 - 2013/8/16
N2 - Background:Obesity has been associated with a more severe disease course in inflammatory bowel disease (IBD) and epidemiological data identified dietary fats but not obesity as risk factors for the development of IBD. Crohn's disease is one of the two major IBD phenotypes and mostly affects the terminal ileum. Despite recent observations that high fat diets (HFD) impair intestinal barrier functions and drive pathobiont selection relevant for chronic inflammation in the colon, mechanisms of high fat diets in the pathogenesis of Crohn's disease are not known. The aim of this study was to characterize the effect of HFD on the development of chronic ileal inflammation in a murine model of Crohn's disease-like ileitis.Methods:TNFΔARE/WT mice and wildtype C57BL/6 littermates were fed a HFD compared to control diet for different durations. Intestinal pathology and metabolic parameters (glucose tolerance, mesenteric tissue characteristics) were assessed. Intestinal barrier integrity was characterized at different levels including polyethylene glycol (PEG) translocation, endotoxin in portal vein plasma and cellular markers of barrier function. Inflammatory activation of epithelial cells as well as immune cell infiltration into ileal tissue were determined and related to luminal factors.Results:HFD aggravated ileal inflammation but did not induce significant overweight or typical metabolic disorders in TNFΔARE/WT. Expression of the tight junction protein Occludin was markedly reduced in the ileal epithelium of HFD mice independently of inflammation, and translocation of endotoxin was increased. Epithelial cells showed enhanced expression of inflammation-related activation markers, along with enhanced luminal factors-driven recruitment of dendritic cells and Th17-biased lymphocyte infiltration into the lamina propria.Conclusions:HFD feeding, independently of obesity, accelerated disease onset of small intestinal inflammation in Crohn's disease-relevant mouse model through mechanisms that involve increased intestinal permeability and altered luminal factors, leading to enhanced dendritic cell recruitment and promoted Th17 immune responses.
AB - Background:Obesity has been associated with a more severe disease course in inflammatory bowel disease (IBD) and epidemiological data identified dietary fats but not obesity as risk factors for the development of IBD. Crohn's disease is one of the two major IBD phenotypes and mostly affects the terminal ileum. Despite recent observations that high fat diets (HFD) impair intestinal barrier functions and drive pathobiont selection relevant for chronic inflammation in the colon, mechanisms of high fat diets in the pathogenesis of Crohn's disease are not known. The aim of this study was to characterize the effect of HFD on the development of chronic ileal inflammation in a murine model of Crohn's disease-like ileitis.Methods:TNFΔARE/WT mice and wildtype C57BL/6 littermates were fed a HFD compared to control diet for different durations. Intestinal pathology and metabolic parameters (glucose tolerance, mesenteric tissue characteristics) were assessed. Intestinal barrier integrity was characterized at different levels including polyethylene glycol (PEG) translocation, endotoxin in portal vein plasma and cellular markers of barrier function. Inflammatory activation of epithelial cells as well as immune cell infiltration into ileal tissue were determined and related to luminal factors.Results:HFD aggravated ileal inflammation but did not induce significant overweight or typical metabolic disorders in TNFΔARE/WT. Expression of the tight junction protein Occludin was markedly reduced in the ileal epithelium of HFD mice independently of inflammation, and translocation of endotoxin was increased. Epithelial cells showed enhanced expression of inflammation-related activation markers, along with enhanced luminal factors-driven recruitment of dendritic cells and Th17-biased lymphocyte infiltration into the lamina propria.Conclusions:HFD feeding, independently of obesity, accelerated disease onset of small intestinal inflammation in Crohn's disease-relevant mouse model through mechanisms that involve increased intestinal permeability and altered luminal factors, leading to enhanced dendritic cell recruitment and promoted Th17 immune responses.
UR - http://www.scopus.com/inward/record.url?scp=84882629237&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0071661
DO - 10.1371/journal.pone.0071661
M3 - Article
C2 - 23977107
AN - SCOPUS:84882629237
SN - 1932-6203
VL - 8
JO - PLoS ONE
JF - PLoS ONE
IS - 8
M1 - e71661
ER -
