High enrichment of prion protein-PrPSc-from BSE infected brain homogenates by adsorptive bubble separation (ABS)

Simon Berner; Albrecht Friess; Perihan Ekici; Harun Parlar

High enrichment of prion protein-PrP^Sc-from BSE infected brain homogenates by adsorptive bubble separation (ABS)

Simon Berner, Albrecht Friess, Perihan Ekici, Harun Parlar

Chair of Food Chemistry and Molecular Sensory Science

Technical University of Munich

Research output: Contribution to journal › Article › peer-review

Abstract

A new enrichment method for prion protein (PrP^Sc) from cattle brain on the basis of adsorptive bubble separation(ABS) using sodium dodecylsulfate (SDS) as surface active component was developed. The obtained results demonstrate, that prion protein (PrP^Sc) could be enriched 140 fold in the foam phase and determinate therefore very sensitive with officially accreditated rapid assay tests. By this way very weak contaminated brains, which lead to negative results in the routine analysis, could be positively tested.

Original language	English
Pages (from-to)	2948-2952
Number of pages	5
Journal	Fresenius Environmental Bulletin
Volume	21
Issue number	10
State	Published - 2012

Keywords

Adsorptive bubble separation
BSE
ELISA
Prions

Cite this

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abstract = "A new enrichment method for prion protein (PrPSc) from cattle brain on the basis of adsorptive bubble separation(ABS) using sodium dodecylsulfate (SDS) as surface active component was developed. The obtained results demonstrate, that prion protein (PrPSc) could be enriched 140 fold in the foam phase and determinate therefore very sensitive with officially accreditated rapid assay tests. By this way very weak contaminated brains, which lead to negative results in the routine analysis, could be positively tested.",

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AU - Ekici, Perihan

AU - Parlar, Harun

PY - 2012

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AB - A new enrichment method for prion protein (PrPSc) from cattle brain on the basis of adsorptive bubble separation(ABS) using sodium dodecylsulfate (SDS) as surface active component was developed. The obtained results demonstrate, that prion protein (PrPSc) could be enriched 140 fold in the foam phase and determinate therefore very sensitive with officially accreditated rapid assay tests. By this way very weak contaminated brains, which lead to negative results in the routine analysis, could be positively tested.

KW - Adsorptive bubble separation

KW - BSE

KW - ELISA

KW - Prions

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ER -

High enrichment of prion protein-PrP^Sc-from BSE infected brain homogenates by adsorptive bubble separation (ABS)

Abstract

Keywords

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