TY - JOUR
T1 - Heterosis for biomass-related traits in arabidopsis investigated by quantitative trait loci analysis of the triple testcross design with recombinant inbred lines
AU - Kusterer, Barbara
AU - Piepho, Hans Peter
AU - Utz, H. Friedrich
AU - Schön, Chris C.
AU - Muminovic, Jasmina
AU - Meyer, Rhonda C.
AU - Altmann, Thomas
AU - Melchinger, Albrecht E.
PY - 2007/11
Y1 - 2007/11
N2 - Arabidopsis thaliana has emerged as a leading model species in plant genetics and functional genomics including research on the genetic causes of heterosis. We applied a triple testcross (TTC) design and a novel biometrical approach to identify and characterize quantitative trait loci (QTL) for heterosis of five biomass-related traits by (i) estimating the number, genomic positions, and genetic effects of heterotic QTL, (ii) characterizing their mode of gene action, and (iii) testing for presence of epistatic effects by a genomewide scan and marker X marker interactions. In total, 234 recombinant inbred lines (RILs) of Arabidopsis hybrid C24 X Col-0 were crossed to both parental lines and their F1 and analyzed with 110 single-nucleotide polymorphism (SNP) markers. QTLanalyses were conducted using linear transformations Z1, Z2, and Z3 calculated from the adjusted entry means of TTC progenies. With Z1, we detected 12 QTL displaying augmented additive effects. With Z2, we mapped six QTL for augmented dominance effects. A one-dimensional genome scan with Z 3 revealed two genomic regions with significantly negative dominance X additive epistatic effects. Two-way analyses of variance between marker pairs revealed nine digenic epistatic interactions: six reflecting dominance X dominance effects with variable sign and three reflecting additive X additive effects with positive sign. We conclude that heterosis for biomass-related traits in Arabidopsis has a polygenic basis with overdominance and/or epistasis being presumably the main types of gene action.
AB - Arabidopsis thaliana has emerged as a leading model species in plant genetics and functional genomics including research on the genetic causes of heterosis. We applied a triple testcross (TTC) design and a novel biometrical approach to identify and characterize quantitative trait loci (QTL) for heterosis of five biomass-related traits by (i) estimating the number, genomic positions, and genetic effects of heterotic QTL, (ii) characterizing their mode of gene action, and (iii) testing for presence of epistatic effects by a genomewide scan and marker X marker interactions. In total, 234 recombinant inbred lines (RILs) of Arabidopsis hybrid C24 X Col-0 were crossed to both parental lines and their F1 and analyzed with 110 single-nucleotide polymorphism (SNP) markers. QTLanalyses were conducted using linear transformations Z1, Z2, and Z3 calculated from the adjusted entry means of TTC progenies. With Z1, we detected 12 QTL displaying augmented additive effects. With Z2, we mapped six QTL for augmented dominance effects. A one-dimensional genome scan with Z 3 revealed two genomic regions with significantly negative dominance X additive epistatic effects. Two-way analyses of variance between marker pairs revealed nine digenic epistatic interactions: six reflecting dominance X dominance effects with variable sign and three reflecting additive X additive effects with positive sign. We conclude that heterosis for biomass-related traits in Arabidopsis has a polygenic basis with overdominance and/or epistasis being presumably the main types of gene action.
UR - http://www.scopus.com/inward/record.url?scp=37249047891&partnerID=8YFLogxK
U2 - 10.1534/genetics.107.077628
DO - 10.1534/genetics.107.077628
M3 - Article
C2 - 18039885
AN - SCOPUS:37249047891
SN - 0016-6731
VL - 177
SP - 1839
EP - 1850
JO - Genetics
JF - Genetics
IS - 3
ER -