Herbinix hemicellulosilytica gen. Nov, sp. nov, a thermophilic cellulose-degrading bacterium isolated from a thermophilic biogas reactor

Daniela E. Koeck, Wolfgang Ludwig, Gerhard Wanner, Vladimir V. Zverlov, Wolfgang Liebl, Wolfgang H. Schwarz

Research output: Contribution to journalArticlepeer-review

65 Scopus citations

Abstract

Phenotypic and phylogenetic studies were performed on new isolates of a novel Gram-stain-positive, anaerobic, non-sporulating, rod-shaped bacterium isolated from a thermophilic biogas plant. The novel organisms were able to degrade crystalline cellulose. 16S rRNA gene comparative sequence analysis demonstrated that the isolates formed a hitherto unknown subline within the family Lachnospiraceae. As a representative of the whole group of isolates, strain T3/55T was further characterized. The closest relative of T3/55T among the taxa with validly published names is Mobilitalea sibirica, sharing 93.9 % 16S rRNA gene sequence similarity. Strain T3/55T was catalase-negative, indole-negative, and produced acetate, ethanol and propionic acid as major end products from cellulose metabolism. The major cellular fatty acids (>1 %) were 16:0 dimethyl acetal, 16:0 fatty acid methyl ester and 16:0 aldehyde. The DNA G + C content was 36.6 mol%. A novel genus and species, Herbinix hemicellulosilytica gen. nov, sp. nov, is proposed based on phylogenetic analysis and physiological properties of the novel isolate. Strain T3/55T (=DSM 29228T =CECT 8801T), represents the type strain of Herbinix hemicellulosilytica gen. nov, sp. nov.

Original languageEnglish
Pages (from-to)2365-2371
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume65
Issue number8
DOIs
StatePublished - 1 Aug 2015
Externally publishedYes

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