Hepatitis B virus-infected HepG2hNTCP cells serve as a novel immunological tool to analyze the antiviral efficacy of CD8+ T cells in vitro

Alexander Hoh; Maximilian Heeg; Yi Ni; Anita Schuch; Benedikt Binder; Nadine Hennecke; Hubert E. Blum; Michael Nassal; Ulrike Protzer; Maike Hofmann; Stephan Urban; Robert Thimme

doi:10.1128/JVI.00605-15

Hepatitis B virus-infected HepG2^hNTCP cells serve as a novel immunological tool to analyze the antiviral efficacy of CD8⁺ T cells in vitro

Alexander Hoh, Maximilian Heeg, Yi Ni, Anita Schuch, Benedikt Binder, Nadine Hennecke, Hubert E. Blum, Michael Nassal, Ulrike Protzer, Maike Hofmann, Stephan Urban, Robert Thimme

Chair of Virology

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27 Scopus citations

Abstract

CD8⁺ T cells are the main effector lymphocytes in the control of hepatitis B virus (HBV) infection. However, limitations of model systems, such as low infection rates, restrict mechanistic studies of HBV-specific CD8⁺ T cells. Here, we established a novel immunological cell culture model based on HBV-infected HepG2^hNTCP cells that endogenously processed viral antigens and presented them to HBV-specific CD8⁺ T cells. This induced cytolytic and noncytolytic CD8⁺ T-cell effector functions and reduction of viral loads.

Original language	English
Pages (from-to)	7433-7438
Number of pages	6
Journal	Journal of Virology
Volume	89
Issue number	14
DOIs	https://doi.org/10.1128/JVI.00605-15
State	Published - 2015

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title = "Hepatitis B virus-infected HepG2hNTCP cells serve as a novel immunological tool to analyze the antiviral efficacy of CD8+ T cells in vitro",

abstract = "CD8+ T cells are the main effector lymphocytes in the control of hepatitis B virus (HBV) infection. However, limitations of model systems, such as low infection rates, restrict mechanistic studies of HBV-specific CD8+ T cells. Here, we established a novel immunological cell culture model based on HBV-infected HepG2hNTCP cells that endogenously processed viral antigens and presented them to HBV-specific CD8+ T cells. This induced cytolytic and noncytolytic CD8+ T-cell effector functions and reduction of viral loads.",

author = "Alexander Hoh and Maximilian Heeg and Yi Ni and Anita Schuch and Benedikt Binder and Nadine Hennecke and Blum, {Hubert E.} and Michael Nassal and Ulrike Protzer and Maike Hofmann and Stephan Urban and Robert Thimme",

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doi = "10.1128/JVI.00605-15",

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T1 - Hepatitis B virus-infected HepG2hNTCP cells serve as a novel immunological tool to analyze the antiviral efficacy of CD8+ T cells in vitro

AU - Hoh, Alexander

AU - Heeg, Maximilian

AU - Ni, Yi

AU - Schuch, Anita

AU - Binder, Benedikt

AU - Hennecke, Nadine

AU - Blum, Hubert E.

AU - Nassal, Michael

AU - Protzer, Ulrike

AU - Hofmann, Maike

AU - Urban, Stephan

AU - Thimme, Robert

PY - 2015

Y1 - 2015

N2 - CD8+ T cells are the main effector lymphocytes in the control of hepatitis B virus (HBV) infection. However, limitations of model systems, such as low infection rates, restrict mechanistic studies of HBV-specific CD8+ T cells. Here, we established a novel immunological cell culture model based on HBV-infected HepG2hNTCP cells that endogenously processed viral antigens and presented them to HBV-specific CD8+ T cells. This induced cytolytic and noncytolytic CD8+ T-cell effector functions and reduction of viral loads.

AB - CD8+ T cells are the main effector lymphocytes in the control of hepatitis B virus (HBV) infection. However, limitations of model systems, such as low infection rates, restrict mechanistic studies of HBV-specific CD8+ T cells. Here, we established a novel immunological cell culture model based on HBV-infected HepG2hNTCP cells that endogenously processed viral antigens and presented them to HBV-specific CD8+ T cells. This induced cytolytic and noncytolytic CD8+ T-cell effector functions and reduction of viral loads.

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AN - SCOPUS:84931052762

SN - 0022-538X

VL - 89

SP - 7433

EP - 7438

JO - Journal of Virology

JF - Journal of Virology

IS - 14

ER -

Hepatitis B virus-infected HepG2^hNTCP cells serve as a novel immunological tool to analyze the antiviral efficacy of CD8⁺ T cells in vitro

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