TY - JOUR
T1 - Helicobacter pylori in autoimmune pancreatitis and pancreatic carcinoma
AU - Jesnowski, Ralf
AU - Isaksson, Bengt
AU - Möhrcke, Christiane
AU - Bertsch, Claus
AU - Bulajic, Milutin
AU - Schneider-Brachert, Wulff
AU - Klöppel, Günter
AU - Lowenfels, Albert B.
AU - Maisonneuve, Patrick
AU - Löhr, J. Matthias
N1 - Funding Information:
This work was supported in part by the Dr. Hans und Lore Graf-Stiftung. We thank Jörg Kleeff for providing us with the fresh frozen tissue. The technical assistance of Annette Funk and Svetlana Sander-Naderi is gratefully acknowledged. Dr. Lowen-fels has received support from Solvay Pharmaceuticals Inc.
PY - 2010/10
Y1 - 2010/10
N2 - Background:Helicobacter pylori has been suggested to be involved in pancreatic diseases, namely autoimmune pancreatitis and pancreatic carcinoma. We investigated the presence of conserved sequences of Helicobacter in pancreatic tissue and pancreatic juice from patients with chronic nonautoimmune and autoimmune pancreatitis as well as pancreatic ductal adenocarcinoma (PDAC). Methods: 35 pancreatic juices collected during routine endoscopic retrograde cholangiopancreatography and 30 pancreatic tissues were studied. Nested PCR was used to detect H. pylori in the isolated DNA samples. In order to exclude a methodological bias, the samples were analyzed blindly in 2 different laboratories using either conventional or LightCycler PCR for H. pylori urease A and 16S ribosomal DNA. Results: In the pancreas of 11 patients with autoimmune pancreatitis, no H. pylori DNA could be detected. Further, in none of the other tissue samples of chronic pancreatitis or PDAC could we detect any Helicobacter sequences. Out of the pancreatic juice samples, none demonstrated either of the 2 Helicobacter gene sequences investigated. Conclusion: Despite good scientific reasoning for an involvement of Helicobacter in pancreatic diseases, a direct infection of the microbial agent seems unlikely. Rather, the pathomechanism must involve molecular mimicry in autoimmune pancreatitis, or the transformation of nitric food constituents to nitrosamines in pancreatic cancer.
AB - Background:Helicobacter pylori has been suggested to be involved in pancreatic diseases, namely autoimmune pancreatitis and pancreatic carcinoma. We investigated the presence of conserved sequences of Helicobacter in pancreatic tissue and pancreatic juice from patients with chronic nonautoimmune and autoimmune pancreatitis as well as pancreatic ductal adenocarcinoma (PDAC). Methods: 35 pancreatic juices collected during routine endoscopic retrograde cholangiopancreatography and 30 pancreatic tissues were studied. Nested PCR was used to detect H. pylori in the isolated DNA samples. In order to exclude a methodological bias, the samples were analyzed blindly in 2 different laboratories using either conventional or LightCycler PCR for H. pylori urease A and 16S ribosomal DNA. Results: In the pancreas of 11 patients with autoimmune pancreatitis, no H. pylori DNA could be detected. Further, in none of the other tissue samples of chronic pancreatitis or PDAC could we detect any Helicobacter sequences. Out of the pancreatic juice samples, none demonstrated either of the 2 Helicobacter gene sequences investigated. Conclusion: Despite good scientific reasoning for an involvement of Helicobacter in pancreatic diseases, a direct infection of the microbial agent seems unlikely. Rather, the pathomechanism must involve molecular mimicry in autoimmune pancreatitis, or the transformation of nitric food constituents to nitrosamines in pancreatic cancer.
KW - Autoimmune pancreatitis
KW - Chronic pancreatitis
KW - Helicobacter pylori
KW - Pancreatic carcinoma
KW - Pancreatic juice
UR - http://www.scopus.com/inward/record.url?scp=77955624210&partnerID=8YFLogxK
U2 - 10.1159/000264677
DO - 10.1159/000264677
M3 - Article
AN - SCOPUS:77955624210
SN - 1424-3903
VL - 10
SP - 462
EP - 466
JO - Pancreatology
JF - Pancreatology
IS - 4
ER -
