Abstract
Background: Head and neck squamous cell carcinoma (HNSCC) are infiltrated by various kinds of immune cells, which show massively impaired immune functions. The influence of HNSCC on CD34 + progenitor cells from human cord blood was analyzed. Materials and Methods: CD34+ cells were isolated from human cord blood by 'magnetic bead separation' using magnetically labelled antibodies. Immunofluorescent staining of CD34 + cells in solid HNSCC was carried out. Cytokine levels of IL-6, IL-8, and IL-10 were analyzed with flow cytometry using the BD CBA Human Soluble Protein Flex Set system (Becton Dickinson). Results: We demonstrated that HNSCC triggered CD34+ cells to produce increased levels of the tumor-promoting cytokine IL-6 and thus they participate in the development of the microenvironment of head and neck cancer. Conclusion: HNSCC modulates the cytokine secretion profile of tumor infiltrating cells to escape from efficient immune responses und to trigger its own malignant progression.
| Original language | English |
|---|---|
| Pages (from-to) | 493-498 |
| Number of pages | 6 |
| Journal | In Vivo |
| Volume | 21 |
| Issue number | 3 |
| State | Published - 2007 |
| Externally published | Yes |
Keywords
- CD34
- HNSCC
- IL-6
- Immunomodulation
- Stem cell
