Growth enhancement of grapevine callus by catechin on auxin-free media

W. Feucht, D. Treutter, I. Keukenkamp

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Proliferating Spatburgunder cells cultured in vitro were found to synthesize the monomeric and oligomeric flavanols catechin, C2, B3, B1, B2 prodelphinidin, ECG (epicatechin gallate), B2G, B2-gallate and B5. Catechin and prodelphinidin were the predominating compounds of all calli studied. Growth promotion of calli from Spatburgunder and Romulus was studied using MS media containing benzyladenine (BA) alone (control), BA plus catechin, and BA combined with two different concentrations of indole-3-acetic acid (IAA). Growth on BA alone was notably reduced. Catechin (68 μM) stimulated callus growth several-fold when combined with 0.8 μM BA; however, this effect was only observed with explants excised from internodes of vigorously growing young shoots.

Original languageEnglish
Pages (from-to)67-71
Number of pages5
JournalVitis
Volume37
Issue number2
StatePublished - Jun 1998

Keywords

  • Callus
  • Catechin
  • Flavanols
  • Grapevine
  • Growth promotion
  • In vitro culture

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