Growth and in situ detection of a pathogenic Escherichia coli in biofilms of a heterotrophic water‐bacterium by use of 16S‐ and 23S‐rRNA‐ directed fluorescent oligonucleotide probes

Ulrich Szewzyk, Werner Manz, Rudolf Amann, K. ‐H Schleifer, Thor‐Axel ‐A Stenström

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

Abstract The colonization of biofilms of a benzoate‐degrading Gram‐positive water bacterium, strain B4, by a pathogenic Escherichia coli was studied in a continuous flow reactor. E. coli added to a fixed bed reactor colonized by B4, was able to grow in the biofilms and subsequently re‐enter the free water phase in high numbers. Mixed biofilms of strain B4 and E. coli were also grown on glass slides for detailed examination of the spatial order of the mixed population biofilm. Individual cells as well as microcolonies of E. coli were detected in the biofilms by hybridization with a fluorescently labeled 23S rRNA‐targeted oligonucleotide probe. The spatial distribution of E. coli could be analyzed in all layers of even thick biofilms.

Original languageEnglish
Pages (from-to)169-176
Number of pages8
JournalFEMS Microbiology Ecology
Volume13
Issue number3
DOIs
StatePublished - Jan 1994

Keywords

  • Biofilm
  • Coliform bacteria
  • Colonization
  • Escherichia coli
  • Oligonucleotide probes

Fingerprint

Dive into the research topics of 'Growth and in situ detection of a pathogenic Escherichia coli in biofilms of a heterotrophic water‐bacterium by use of 16S‐ and 23S‐rRNA‐ directed fluorescent oligonucleotide probes'. Together they form a unique fingerprint.

Cite this