Glycosylation analysis of gel-separated proteins

Bernhard Küster, Thomas N. Krogh, Ejvind Mørtz, David J. Harvey

Research output: Contribution to journalReview articlepeer-review

88 Scopus citations

Abstract

Beyond the identification of proteins involved in a particular physiological situation, many aspects of proteomics require more detailed characterization of the proteins involved. Post-translational modifications (PTMs) of proteins are a common means to target proteins, regulate their activities and to mediate communication between proteins and cells. Owing to the much higher analytical complexity of glycan analysis compared to e.g. protein identification, PTM analysis in general and glycosylation analysis in particular is largely neglected in proteomics. In this review, the current technological status of global and site-specific glycosylation analysis of gel-separated proteins is described and the way in which the available technology can be employed in proteomics is critically discussed.

Original languageEnglish
Pages (from-to)350-361
Number of pages12
JournalProteomics
Volume1
Issue number2
DOIs
StatePublished - Feb 2001
Externally publishedYes

Keywords

  • Gel electrophoresis
  • Glycosylation
  • Mass spectrometry
  • Protein
  • Proteomics
  • Review
  • Sequencing

Fingerprint

Dive into the research topics of 'Glycosylation analysis of gel-separated proteins'. Together they form a unique fingerprint.

Cite this