Glycolytic ATP production estimated from31P magnetic resonance spectroscopy measurements during ischemic exercise in vivo

H. Wackerhage, K. Mueller, U. Hoffmann, D. Leyk, D. Essfeld, J. Zange

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

In an oxygen-depleted muscle, glycolytically produced ATP is inversely related to the ([ATP] + creatine phosphate [PCr]) decrease because ATP, PCr, and glycolysis are virtually the only energy sources under these conditions. In particular, the onset of glycolysis or any appreciable increase in the rate of glycolytic ATP production will lead to a slower rate of ([ATP] + [PCr]) breakdown at a given energy consumption. To quantify this relationship, endurance athletes performed isometric foot plantar flexion (20% of a test force [TF], n = 10; 50% TF, n = 5) during local arterial occlusion. Parameters of energy metabolism were measured with 31P magnetic resonance spectroscopy (31P-MRS). During exercise, [PCr] decreased to 80 ± 10 (20% TF) and 11 ± 4% (50% TF) of its resting concentration, and pH dropped from 7.04 ± 0.01 to 6.98 ± 0.10 (20% TF) and from 7.03 ± 0.02 to 6.70 ± 0.10 (50% TF). In both experiments, two phases of ([ATP] + [PCr]) decrease were observed: an initial faster decrease was followed by a slower decline. The latter phase started at about the time when the pH began to drop. The difference between a line extrapolated from the slope of the initial phase and the measured ([ATP] + [PCr]) decrease was used as an estimate for glycolytically produced ATP. This estimate and pH were significantly correlated with r = -0.97 (20% TF) and r = -0.99 (50% TF), These results indicate that glycolytically produced ATP can be estimated from the ([ATP] + [PCr]) decrease during exercise.

Original languageEnglish
Pages (from-to)151-155
Number of pages5
JournalMagnetic Resonance Materials in Physics, Biology and Medicine
Volume4
Issue number3-4
DOIs
StatePublished - 1996
Externally publishedYes

Keywords

  • Exercise
  • Glycolysis
  • Ischemia
  • P magnetic resonance spectroscopy
  • Phosphofructokinase (EC 2.7.1.11)
  • Skeletal muscle

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