Germination of Pistacia vera L. pollen in liquid medium

A. Golan-Goldhirsh, U. Schmidhalter, M. Müller, J. J. Oertli

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12 Scopus citations

Abstract

The osmotic effect of Polyethylene glycol (PEG) has been shown to be sufficient to induce the germination of Pistacia vera L. pollen in liquid medium. The prehydration of the pollen in a saturated atmosphere for approximately 10 h was necessary to obtain maximum in vitro germination. Imbibition of the pollen in water resulted in the rapid leakage of solutes into the medium. These solutes consisted of approximately 50% carbohydrates, of which sucrose (0.65 μmol/mg), glucose (0.77 μmol/mg) and fructose (0.78 μmol/mg) were the major sugars; the remaining 50% comprised proteins with the following major molecular weights 63 kDa, 60 kDa, 59 kDa, 40 kDa, 36 kDa, 35.5 kDa, 31 kDa, other organic matter and minerals.

Original languageEnglish
Pages (from-to)182-187
Number of pages6
JournalSexual Plant Reproduction
Volume4
Issue number3
DOIs
StatePublished - Jul 1991
Externally publishedYes

Keywords

  • In vitro germination
  • Osmotic effect
  • Pistacia vera L
  • Pollen

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