Genomic complexities of murine leukemia and sarcoma, reticuloendotheliosis, and visna viruses

K. L. Beemon; A. J. Faras; A. T. Haase

doi:10.1128/jvi.17.2.525-537.1976

Genomic complexities of murine leukemia and sarcoma, reticuloendotheliosis, and visna viruses

K. L. Beemon
, A. J. Faras
, A. T. Haase

University of California at Berkeley

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

The genetic complexities of several ribodeoxyviruses were measured by quantitative analysis of unique RNase T₁ resistant oligonucleotides from 60-70S viral RNAs. Moloney murine leukemia virus was found to have an RNA complexity of 3.5x10⁶ daltons, whereas Moloney murine sarcoma virus had a significantly smaller genome size of 2.3x10⁶. Reticuloendotheliosis and visna virus RNAs had complexities of 3.9x10⁶ and 3.5x10⁶, respectively. Analysis of RNase A resistant oligonucleotides of Rous sarcoma virus RNA gave a complexity of 3.6x10⁶, similar to that previously obtained with RNase T₁ resistant oligonucleotides. Since each of these viruses was found to have a unique sequence genomic complexity near the molecular weight of a single 30-40S viral RNA sub unit, it was concluded that ribodeoxyvirus genomes are at least largely polypoid.

Original language	English
Pages (from-to)	525-537
Number of pages	13
Journal	Journal of Virology
Volume	17
Issue number	2
DOIs	https://doi.org/10.1128/jvi.17.2.525-537.1976
State	Published - 1976
Externally published	Yes

Access to Document

10.1128/jvi.17.2.525-537.1976

Cite this

@article{36608a791cf8414095af796e35d1c926,

title = "Genomic complexities of murine leukemia and sarcoma, reticuloendotheliosis, and visna viruses",

abstract = "The genetic complexities of several ribodeoxyviruses were measured by quantitative analysis of unique RNase T1 resistant oligonucleotides from 60-70S viral RNAs. Moloney murine leukemia virus was found to have an RNA complexity of 3.5x106 daltons, whereas Moloney murine sarcoma virus had a significantly smaller genome size of 2.3x106. Reticuloendotheliosis and visna virus RNAs had complexities of 3.9x106 and 3.5x106, respectively. Analysis of RNase A resistant oligonucleotides of Rous sarcoma virus RNA gave a complexity of 3.6x106, similar to that previously obtained with RNase T1 resistant oligonucleotides. Since each of these viruses was found to have a unique sequence genomic complexity near the molecular weight of a single 30-40S viral RNA sub unit, it was concluded that ribodeoxyvirus genomes are at least largely polypoid.",

author = "Beemon, \{K. L.\} and Faras, \{A. J.\} and Haase, \{A. T.\}",

year = "1976",

doi = "10.1128/jvi.17.2.525-537.1976",

language = "English",

volume = "17",

pages = "525--537",

journal = "Journal of Virology",

issn = "0022-538X",

publisher = "American Society for Microbiology",

number = "2",

}

TY - JOUR

T1 - Genomic complexities of murine leukemia and sarcoma, reticuloendotheliosis, and visna viruses

AU - Beemon, K. L.

AU - Faras, A. J.

AU - Haase, A. T.

PY - 1976

Y1 - 1976

N2 - The genetic complexities of several ribodeoxyviruses were measured by quantitative analysis of unique RNase T1 resistant oligonucleotides from 60-70S viral RNAs. Moloney murine leukemia virus was found to have an RNA complexity of 3.5x106 daltons, whereas Moloney murine sarcoma virus had a significantly smaller genome size of 2.3x106. Reticuloendotheliosis and visna virus RNAs had complexities of 3.9x106 and 3.5x106, respectively. Analysis of RNase A resistant oligonucleotides of Rous sarcoma virus RNA gave a complexity of 3.6x106, similar to that previously obtained with RNase T1 resistant oligonucleotides. Since each of these viruses was found to have a unique sequence genomic complexity near the molecular weight of a single 30-40S viral RNA sub unit, it was concluded that ribodeoxyvirus genomes are at least largely polypoid.

AB - The genetic complexities of several ribodeoxyviruses were measured by quantitative analysis of unique RNase T1 resistant oligonucleotides from 60-70S viral RNAs. Moloney murine leukemia virus was found to have an RNA complexity of 3.5x106 daltons, whereas Moloney murine sarcoma virus had a significantly smaller genome size of 2.3x106. Reticuloendotheliosis and visna virus RNAs had complexities of 3.9x106 and 3.5x106, respectively. Analysis of RNase A resistant oligonucleotides of Rous sarcoma virus RNA gave a complexity of 3.6x106, similar to that previously obtained with RNase T1 resistant oligonucleotides. Since each of these viruses was found to have a unique sequence genomic complexity near the molecular weight of a single 30-40S viral RNA sub unit, it was concluded that ribodeoxyvirus genomes are at least largely polypoid.

UR - https://www.scopus.com/pages/publications/0017236461

U2 - 10.1128/jvi.17.2.525-537.1976

DO - 10.1128/jvi.17.2.525-537.1976

M3 - Article

C2 - 176429

AN - SCOPUS:0017236461

SN - 0022-538X

VL - 17

SP - 525

EP - 537

JO - Journal of Virology

JF - Journal of Virology

IS - 2

ER -

Genomic complexities of murine leukemia and sarcoma, reticuloendotheliosis, and visna viruses

Abstract

Access to Document

Other files and links

Fingerprint

Cite this